仅在用紫外线灭活病毒进行预刺激后，用柯萨奇B1病毒RNA显微注射HEp-2细胞才会增强福氏志贺菌的侵袭性。

Microinjection of HEp-2 cells with coxsackie B1 virus RNA enhances invasiveness of Shigella flexneri only after prestimulation with UV-inactivated virus.

作者信息

Modalsli K R, Mikalsen S O, Bukholm G, Degré M

机构信息

Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Norway.

出版信息

APMIS. 1993 Aug;101(8):602-6. doi: 10.1111/j.1699-0463.1993.tb00153.x.

DOI:10.1111/j.1699-0463.1993.tb00153.x

PMID:8217113

Abstract

Coxsackie B1 virus induces increased susceptibility to invasion by Shigella flexneri when HEp-2 cells are inoculated with the complete virus. When RNA from the same virus was microinjected into cells, virus RNA was synthesized and new virus particles were formed, but the transfected RNA had no effect on bacterial invasiveness. However, when the cells were prestimulated with UV-inactivated virus, the microinjected RNA induced an additional enhancement of bacterial invasiveness. Microinjected whole virus particles did not replicate and did not induce any change in bacterial invasiveness. The results indicate that an initial event in virus multiplication is necessary to achieve an effect of transfected viral RNA on invasion of S. flexneri.

摘要

当用完整的柯萨奇B1病毒接种HEp-2细胞时，该病毒会导致细胞对福氏志贺菌的侵袭敏感性增加。当将来自同一病毒的RNA显微注射到细胞中时，会合成病毒RNA并形成新的病毒颗粒，但转染的RNA对细菌的侵袭性没有影响。然而，当用紫外线灭活的病毒对细胞进行预刺激时，显微注射的RNA会进一步增强细菌的侵袭性。显微注射的完整病毒颗粒不会复制，也不会引起细菌侵袭性的任何变化。结果表明，病毒增殖的初始事件对于实现转染的病毒RNA对福氏志贺菌侵袭的影响是必要的。