Lu Fang-hong, Shang Qing, Wen Pei-e, Su Guo-hai, Wu Jian-mei, Tian Qi, Zhao Ying-xin
Department of Cardiovascular Disease, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, Shandong, 250062 P.R.China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Apr;23(2):195-7.
To study the linkage between K469E polymorphism of intercellular adhesion molecule 1(ICAM1) gene with ICAM1 plasma level and coronary heart disease (CHD) in Han population of China.
One hundred and sixty-four controls without CHD and 160 patients with CHD were enrolled in our study. By nested PCR with allele-specific oligonucleotide primers, all patients and controls were genotyped for the ICAM1 polymorphism. And the ICAM1 plasma level was measured by ELISA.
In the patients with CHD, both K allele frequency and the plasma level of ICAM1 were higher than those in control (P<0.05). The individual with K allele had higher plasma level of ICAM1 than that without K allele (344.34+/-128.59 microg/L vs 303.54+/-108.74 microg/L, P=0.008). K allele enhanced the risk of CHD (P<0.01, OR=2.158, 95%CI: 1.250-3.727). There was the K allele cooperation with smoking in influencing the risk of CHD.
There is the polymorphism of ICAM1 K469E gene in Han population of China, and the K allele may be a genetic factor influencing the risk of CHD.
研究中国汉族人群细胞间黏附分子1（ICAM1）基因K469E多态性与ICAM1血浆水平及冠心病（CHD）之间的联系。
本研究纳入了164例无冠心病的对照者和160例冠心病患者。采用等位基因特异性寡核苷酸引物巢式PCR对所有患者和对照者的ICAM1多态性进行基因分型。并采用酶联免疫吸附测定法检测ICAM1血浆水平。
冠心病患者中，K等位基因频率和ICAM1血浆水平均高于对照组（P<0.05）。携带K等位基因的个体ICAM1血浆水平高于不携带K等位基因的个体（344.34±128.59μg/L对303.54±108.74μg/L，P=0.008）。K等位基因增加了冠心病的发病风险（P<0.01，OR=2.158，95%CI：1.250-3.727）。K等位基因与吸烟在影响冠心病风险方面存在协同作用。
中国汉族人群存在ICAM1 K469E基因多态性，K等位基因可能是影响冠心病发病风险的遗传因素。
