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多重及基于微粒的分析:用于生物系统大规模分析的定量工具。

Multiplexed and microparticle-based analyses: quantitative tools for the large-scale analysis of biological systems.

作者信息

Nolan John P, Mandy Francis

机构信息

La Jolla Bioengineering Institute, 505 Coast Boulevard South, La Jolla, California 92037, USA.

出版信息

Cytometry A. 2006 May;69(5):318-25. doi: 10.1002/cyto.a.20266.

Abstract

While the term flow cytometry refers to the measurement of cells, the approach of making sensitive multiparameter optical measurements in a flowing sample stream is a very general analytical approach. The past few years have seen an explosion in the application of flow cytometry technology for molecular analysis and measurements using microparticles as solid supports. While microsphere-based molecular analyses using flow cytometry date back three decades, the need for highly parallel quantitative molecular measurements that has arisen from various genomic and proteomic advances has driven the development in particle encoding technology to enable highly multiplexed assays. Multiplexed particle-based immunoassays are now common place, and new assays to study genes, protein function, and molecular assembly. Numerous efforts are underway to extend the multiplexing capabilities of microparticle-based assays through new approaches to particle encoding and analyte reporting. The impact of these developments will be seen in the basic research and clinical laboratories, as well as in drug development.

摘要

虽然流式细胞术一词指的是对细胞的测量,但在流动样本流中进行灵敏的多参数光学测量的方法是一种非常通用的分析方法。在过去几年中,流式细胞术技术在使用微粒作为固体支持物进行分子分析和测量方面的应用激增。虽然基于微球的流式细胞术分子分析可追溯到三十年前,但各种基因组学和蛋白质组学进展所引发的对高度平行定量分子测量的需求推动了粒子编码技术的发展,以实现高度多重分析。基于多重粒子的免疫分析现在很常见,还有用于研究基因、蛋白质功能和分子组装的新分析方法。目前正在进行大量努力,通过粒子编码和分析物报告的新方法来扩展基于微粒的分析的多重能力。这些进展的影响将在基础研究和临床实验室以及药物开发中显现出来。

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