Department of Chemistry, California State University, Fresno, CA 93750, United States; Department of Medical Pathology and Laboratory Medicine, University of California School of Medicine, Sacramento, CA 95817, United States.
Omni Array Biotechnology, Rockville, MD 20855, United States.
J Immunol Methods. 2018 Oct;461:1-14. doi: 10.1016/j.jim.2018.07.002. Epub 2018 Jul 10.
Advances in high-throughput proteomic approaches have provided substantial momentum to novel disease-biomarker discovery research and have augmented the quality of clinical studies. Applications based on multiplexed microsphere suspension array technology are making strong in-roads into the clinical diagnostic/prognostic practice. Conventional proteomic approaches are designed to discover a broad set of proteins that are associated with a specific medical condition. In comparison, multiplex microsphere immunoassays use quantitative measurements of selected set(s) of specific/particular molecular markers such as cytokines, chemokines, pathway signaling or disease-specific markers for detection, metabolic disorders, cancer, and infectious agents causing human, plant and animal diseases. This article provides a foundation to the multiplexed microsphere suspension array technology, with an emphasis on the improvements in the technology, data analysis approaches, and applications to translational and clinical research with implications for personalized and precision medicine.
高通量蛋白质组学方法的进步为新的疾病生物标志物发现研究提供了巨大动力，并提高了临床研究的质量。基于多重微球悬浮阵列技术的应用正在强力进入临床诊断/预后实践。传统的蛋白质组学方法旨在发现与特定医疗状况相关的广泛的蛋白质组。相比之下，多重微球免疫分析用于定量测量选定的一组特定/特定分子标志物，如细胞因子、趋化因子、通路信号或疾病特异性标志物，用于检测代谢紊乱、癌症和引起人类、植物和动物疾病的传染病原体。本文为多重微球悬浮阵列技术提供了基础，重点介绍了该技术的改进、数据分析方法以及在转化和临床研究中的应用，对个性化和精准医疗具有重要意义。
