Niu Yun, Wang Ying, Yu Yong, Ding Xiu-min, Lü Shu-hua, Xiao Xu-qi
Breast Cancer Research Key Laboratory of Education Ministry, Tianjin Cancer Institute and Cancer Hospital, Tianjin Medical University, Tianjin 300060, China.
Zhonghua Yi Xue Za Zhi. 2006 Jan 3;86(1):56-60.
To investigate the expression of alpha-tubulin and gamma-tubulin, 2 kinds of centrosome proteins, in premalignant lesion and carcinoma of breast and the significance thereof.
Forty specimens of premalignant lesions of breast, 40 specimens of infiltrating ductal carcinoma of breast, 40 specimens of intraductal carcinoma (IDC), and 30 specimens of normal breast tissues were obtained during operation. Immunohistochemistry was used to analyze the protein expression of alpha-tubulin and gamma-tubulin, and the percentages of ki67 positive cells. Western blotting was used to examine the mRNA expression of alpha-tubulin and gamma-tubulin.
The protein and mRNA expression values of alpha-tubulin and gamma-tubulin in breast carcinoma were higher than those in the premalignant lesions and normal breast tissues with significant differences between the premalignant lesions and normal breast torques and without significant differences between infiltrating ductal carcinoma of breast and IDC. The ki67 positive rates of the infiltrating ductal carcinoma of breast group, IDC group, premalignant lesion group, and normal breast tissues group were 16.0%, 37.0%, 53.6%, and 67.8% (P = 0.001). A positive correlation existed between the expression of alpha-tubulin and the expression of gamma-tubulin in the same case and the same group (all P = 0.00) and there was no significant correlation between the expression of alpha-tubulin and the expression of gamma-tubulin in the same case and the same group (all P > 0.05). Both the expression of alpha-tubulin and the expression of gamma-tubulin were significantly associated with the prognosis.
Centrosome protein is one of the distinct phenotypes of breast cancer cells. Aberration of centrosome proteins may be used to screen high risk cases of breast carcinoma and to estimate the prognosis.
探讨两种中心体蛋白α-微管蛋白和γ-微管蛋白在乳腺的癌前病变及癌组织中的表达情况及其意义。
手术获取40例乳腺癌前病变标本、40例乳腺浸润性导管癌标本、40例导管内癌（IDC）标本及30例正常乳腺组织标本。采用免疫组织化学法分析α-微管蛋白和γ-微管蛋白的蛋白表达及ki67阳性细胞百分比。采用蛋白质印迹法检测α-微管蛋白和γ-微管蛋白的mRNA表达。
乳腺癌组织中α-微管蛋白和γ-微管蛋白的蛋白及mRNA表达值高于癌前病变及正常乳腺组织，癌前病变与正常乳腺组织间差异有统计学意义，乳腺浸润性导管癌与IDC间差异无统计学意义。乳腺浸润性导管癌组、IDC组、癌前病变组及正常乳腺组织组的ki67阳性率分别为16.0%、37.0%、53.6%及67.8%（P = 0.001）。同一病例同一组中α-微管蛋白表达与γ-微管蛋白表达呈正相关（均P = 0.00），同一病例同一组中α-微管蛋白表达与γ-微管蛋白表达无显著相关性（均P > 0.05）。α-微管蛋白表达和γ-微管蛋白表达均与预后显著相关。
中心体蛋白是乳腺癌细胞的独特表型之一。中心体蛋白异常可用于筛查乳腺癌高危病例及评估预后。
