Key Laboratory of Carbohydrate Chemistry and Biotechnology of National Ministry of Education, and National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi, China.
Pathol Res Pract. 2013 Apr;209(4):221-7. doi: 10.1016/j.prp.2012.12.006. Epub 2013 Mar 21.
The centrosome plays an essential role in chromosomal segregation during cell division. Centrosome dysfunction might lead to aneuploidy and chromosomal instability. Invasive breast tumors with centrosome amplification often show aneuploidy. Flow cytometry (FCM) was used to examine the aneuploidy rate in 30 cases of each of the following seven tissue types: normal breast tissue, usual ductal hyperplasia, atypical ductal hyperplasia, low-grade ductal carcinoma in situ, high-grade ductal carcinoma in situ, low-grade invasive ductal carcinoma, and high-grade invasive ductal carcinoma. Centrosomal α, γ-tubulin expression was examined by FCM immunofluorescence and compared between diploid and aneuploid cells. The aneuploidy rate was 0, 6.7%, 26.7%, 30.0%, 46.7%, 56.7%, and 86.7%, respectively, in the seven tissue types. The percentage of cells expressing α- and γ-tubulins was significantly different between the seven groups, and the positive rate of α- and γ-tubulin expression in ADH, DCIS and IDC was higher than that in NBT and UDH. The percentage of cells expressing α- and γ-tubulins in the diploid state was significantly lower than that in the aneuploid state. Expression of centrosomal α- and γ-tubulins seems to be associated with DNA ploidy in breast premalignant lesions and carcinoma during the progression of breast cancer.
中心体在细胞分裂过程中的染色体分离中起着至关重要的作用。中心体功能障碍可能导致非整倍体和染色体不稳定。具有中心体扩增的浸润性乳腺肿瘤常表现为非整倍体。流式细胞术 (FCM) 用于检查以下七种组织类型中每一种的 30 例样本的非整倍体率：正常乳腺组织、普通导管增生、非典型导管增生、低级别导管原位癌、高级别导管原位癌、低级别浸润性导管癌和高级别浸润性导管癌。通过 FCM 免疫荧光检查中心体 α、γ-微管蛋白的表达，并在二倍体和非整倍体细胞之间进行比较。七种组织类型的非整倍体率分别为 0、6.7%、26.7%、30.0%、46.7%、56.7%和 86.7%。α-和 γ-微管蛋白表达的细胞百分比在这七个组之间有显著差异，ADH、DCIS 和 IDC 中的α-和 γ-微管蛋白的阳性率高于 NBT 和 UDH。二倍体状态下 α-和 γ-微管蛋白表达的细胞百分比明显低于非整倍体状态。中心体α-和γ-微管蛋白的表达似乎与乳腺癌进展过程中乳腺癌前病变和癌的 DNA 倍性有关。
