Andreeva E R, Orekhov A N, Smirnov V N
Institute of Experimental Cardiology, Academy of Medical Sciences, Moscow, USSR.
Acta Anat (Basel). 1991;141(4):316-23. doi: 10.1159/000147142.
The intima of the adult human aorta consists of three sublayers: a muscular layer lying next to the media, a median hyperplastic layer and an innermost connective tissue layer, adjoining the lumen. The cells inhabiting these sublayers were isolated by the method of alcoholic-alkaline dissociation from grossly normal areas, fatty streaks and atherosclerotic plaques. The populations obtained contained cells with different numbers of cytoplasmic inclusions and a number without any. In unaffected intima and in fatty streaks, the cells with lipid inclusions were found predominantly in the outermost intimal layer including the connective tissue and in part of the median hyperplastic layer. In the superficial layer of unaffected intima and the fatty streak, these cells accounted for 15 and 25% of the total cell population, respectively. In the plaque, most cells with lipid inclusions were localized in the median hyperplastic layer of the intima (10%). The muscular layer was characterized by the lowest content of cells with lipid inclusions both in the unaffected intima and atherosclerotic lesions (from 0.75% in unaffected intima to 5% plaques). Among the intimal smooth muscle cells of various shapes, the cells with lipid inclusions were most often found in the stellate cell subpopulation (5-35%). A possible role of stellate cells in atherogenesis is discussed.
紧邻中膜的肌肉层、中间增生层和最内层的结缔组织层，后者毗邻管腔。采用酒精 - 碱性解离方法从大体正常区域、脂肪条纹和动脉粥样硬化斑块中分离出居住在这些亚层中的细胞。获得的细胞群体包含具有不同数量细胞质内含物的细胞以及一些没有任何内含物的细胞。在未受影响的内膜和脂肪条纹中，含有脂质内含物的细胞主要存在于最外层内膜，包括结缔组织和部分中间增生层。在未受影响的内膜和脂肪条纹的表层中，这些细胞分别占细胞总数的15%和25%。在斑块中，大多数含有脂质内含物的细胞位于内膜的中间增生层（10%）。无论是在未受影响的内膜还是动脉粥样硬化病变中，肌肉层中含有脂质内含物的细胞含量最低（从未受影响内膜中的0.75%到斑块中的5%）。在各种形状的内膜平滑肌细胞中，含有脂质内含物的细胞最常出现在星状细胞亚群中（5 - 35%）。本文讨论了星状细胞在动脉粥样硬化发生中的可能作用。
