Ramos-Gonzalez M I, Duque E, Ramos J L
Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Unidad de Bioquímica Vegetal, Granada, Spain.
Appl Environ Microbiol. 1991 Oct;57(10):3020-7. doi: 10.1128/aem.57.10.3020-3027.1991.
Recombinant TOL plasmid pWWO-EB62 allows Pseudomonas putida to grow on p-ethylbenzoate. This plasmid can be transferred to other microorganisms, and its catabolic functions for the metabolism of alkylbenzoates are expressed in a limited number of gram-negative bacteria, including members of pseudomonad rRNA group I and Escherichia coli. Transfer of the recombinant plasmid to Erwinia chrysanthemi was observed, but transconjugants failed to grow on alkylbenzoates because they lost catabolic functions. Pseudomonads belonging to rRNA groups II, III, and IV, Acinetobacter calcoaceticus, and Alcaligenes sp. could not act as recipients for TOL, either because the plasmid was not transferred or because it was not stably maintained. The frequency of transfer of pWWO-EB62 from P. putida as a donor to pseudomonads belonging to rRNA group I was on the order of 1 to 10(-2) transconjugant per recipient, while the frequency of intergeneric transfer ranged from 10(-3) to 10(-7) transconjugant per recipient. The profile of potential hosts was conserved when the donor bacterium was Escherichia coli or Erwinia chrysanthemi instead of P. putida. No intergeneric gene transfer of the recombinant TOL plasmid was observed in soils; however, intraspecies transfer did take place. Intraspecies transfer of TOL in soils was affected by the type of soil used, the initial inoculum size, and the presence of chemicals that could affect the survival of the donor or recipient bacteria.
重组TOL质粒pWWO-EB62使恶臭假单胞菌能够在对乙基苯甲酸上生长。该质粒可转移至其他微生物，其对烷基苯甲酸代谢的分解代谢功能在有限数量的革兰氏阴性菌中表达，包括假单胞菌rRNA第一组的成员和大肠杆菌。观察到重组质粒转移至菊欧文氏菌，但转接合子无法在烷基苯甲酸上生长，因为它们失去了分解代谢功能。属于rRNA第二、第三和第四组的假单胞菌、醋酸钙不动杆菌和产碱菌属不能作为TOL的受体，要么是因为质粒未转移，要么是因为它未稳定维持。从恶臭假单胞菌作为供体向属于rRNA第一组的假单胞菌转移pWWO-EB62的频率约为每受体1至10^(-2)个转接合子，而属间转移频率为每受体10^(-3)至10^(-7)个转接合子。当供体细菌是大肠杆菌或菊欧文氏菌而非恶臭假单胞菌时，潜在宿主的概况是保守的。在土壤中未观察到重组TOL质粒的属间基因转移；然而，种内转移确实发生了。土壤中TOL的种内转移受所用土壤类型、初始接种量以及可能影响供体或受体细菌存活的化学物质的存在影响。
