Bradley D E, Williams P A
J Gen Microbiol. 1982 Dec;128(12):3019-24. doi: 10.1099/00221287-128-12-3019.
Pseudomonas putida mt-2, formerly known as Pseudomonas arvilla mt-2, which carries the wild-type TOL plasmid, and P. putida strain AC37 carrying TOL, were completely lysed by the pilus-adsorbing plasmid-specific bacteriophages PR4 and PRD1. Pseudomonas putida strain PpS388, also harbouring the plasmid, was not lysed. In a P. putida mt-2 host, TOL transferred 18-fold better on a surface (2.5 X 10(-1) transconjugants per donor h-1) than in liquid; when P. putida PpS388 was the host, however, a frequency of only 2.3 X 10(-4) transconjugants per donor h-1 was obtained. Thus, TOL was derepressed for transfer in P. putida mt-2 and P. putida AC37, but not in P. putida PpS388. Electron microscopy revealed that TOL determined thick (8.5-10 nm diameter) flexible pili in large numbers, suggesting constitutive expression in its derepressed state.
恶臭假单胞菌mt-2（以前称为阿氏假单胞菌mt-2）携带野生型TOL质粒，携带TOL的恶臭假单胞菌菌株AC37被菌毛吸附的质粒特异性噬菌体PR4和PRD1完全裂解。同样携带该质粒的恶臭假单胞菌菌株PpS388未被裂解。在恶臭假单胞菌mt-2宿主中，TOL在表面上的转移效率比在液体中高18倍（每供体小时2.5×10⁻¹个转接合子）；然而，当恶臭假单胞菌PpS388作为宿主时，每供体小时仅获得2.3×10⁻⁴个转接合子的频率。因此，TOL在恶臭假单胞菌mt-2和恶臭假单胞菌AC37中转移时去阻遏，但在恶臭假单胞菌PpS388中没有。电子显微镜显示，TOL在大量细胞中产生了直径为8.5 - 10纳米的厚而柔韧的菌毛，这表明在其去阻遏状态下是组成型表达。
