Jeenes D J, Reineke W, Knackmuss H J, Williams P A
J Bacteriol. 1982 Apr;150(1):180-7. doi: 10.1128/jb.150.1.180-187.1982.
WR211 and WR216 are derivatives of halobenzoate-degrading Pseudomonas sp. strain B13 into which the 117-kilobase TOL degradative plasmid pWW0 has been transferred from Pseudomonas putida mt-2. WR211 has lost the ability to grow on the TOL-specific substrate m-xylene but retains the ability to grow on its metabolite, m-toluate. An analysis of the induction of enzymes was consistent with WR211 carrying a nonfunctional regulatory gene, xy1R, WR216 is a spontaneous derivative of WR211 which grows on one of the TOL substrates and yet expresses the nonspecific toluate oxidase, which enables it to grow on the novel substrate 4-chlorobenzoate. In addition to the xy1R lesion inherited from WR211, WR216 appears to carry a mutation in the structural gene for catechol 2,3-oxygenase, xy1E. The plasmids in both strains were analyzed by restriction endonuclease digestion. pWW0-1211 in WR211 has a large deletion (39 kilobases) compared with pWW0 and appears to be identical to a previously described plasmid (pWW0-8) which encodes none of the TOL degradative functions. pWW0-1216 in WR216 has undergone a major structural reorganization relative to its parent, pWW0-1211. This plasmid has a smaller deletion (19 kilobases), which is staggered relative to the deletion in pWW0-1211, and in addition it has two 3-kilobase insertions of unknown origin, one of which appears to cause the xylE mutation.
WR211和WR216是卤代苯甲酸降解假单胞菌属菌株B13的衍生物，117千碱基的TOL降解质粒pWW0已从恶臭假单胞菌mt - 2转移至该菌株中。WR211失去了在TOL特异性底物间二甲苯上生长的能力，但保留了在其代谢产物间甲苯酸上生长的能力。对酶诱导的分析表明，WR211携带一个无功能的调控基因xy1R。WR216是WR211的自发衍生物，它能在一种TOL底物上生长，并且表达非特异性甲苯酸氧化酶，这使其能够在新底物4 - 氯苯甲酸上生长。除了从WR211继承的xy1R损伤外，WR216似乎在儿茶酚2,3 - 双加氧酶的结构基因xy1E中发生了突变。通过限制性内切酶消化分析了这两种菌株中的质粒。与pWW0相比，WR211中的pWW0 - 1211有一个大的缺失（39千碱基），并且似乎与先前描述的一个质粒（pWW0 - 8）相同，该质粒不编码任何TOL降解功能。WR216中的pWW0 - 1216相对于其亲本pWW0 - 1211经历了一次主要的结构重组。该质粒有一个较小的缺失（19千碱基），相对于pWW0 - 1211中的缺失是交错的，此外它还有两个来源不明的3千碱基插入片段，其中一个似乎导致了xylE突变。
