磷酸葡萄糖异构酶催化的己糖磷酸酯的相互转化：通过¹H NMR光谱法区分6-磷酸-D-果糖的1-单氘代同位素异构体

Phosphoglucoisomerase-catalyzed interconversion of hexose phosphates: distinction of the 1-monodeutero-isotopomers of D-fructose 6-phosphate by 1H NMR spectroscopy.

作者信息

Willem R, Malaisse-Lagae F, Malaisse W J

机构信息

Hoog Resolutie NMR-Centrum (HR-NMRC) en Algemene en Organische Scheikunde (AOSC-TW), Vrije Universiteit Brussel, Belgium.

出版信息

Biochimie. 1991 Sep;73(9):1241-4. doi: 10.1016/0300-9084(91)90009-p.

DOI:10.1016/0300-9084(91)90009-p

PMID:1660728

Abstract

The 1H NMR spectrum obtained with the alpha- and beta-anomers of D-[1-2H]fructose 6-phosphate generated from D-glucose 6-phosphate sequentially exposed in D2O to phosphoglucoisomerase, phosphofructokinase and fructose-1,6-diphosphatase differed from that recorded when the deuterated ketohexose phosphate was produced from D-mannose 6-phosphate sequentially exposed in D2O to phosphomannoisomerase, phosphofructokinase and fructose-1,6-diphosphatase. The identification of the 2 isotopomers of D-fructose 6-phosphate by 1H NMR spectroscopy provides a new tool to assess the relative extent of interconversion of hexose phosphates in the reactions catalyzed by phosphoglucoisomerase and phosphomannoisomerase, respectively.

摘要

用D - [1 - 2H]果糖6 - 磷酸的α - 和β - 异头物获得的1H NMR谱，该异头物由D - 葡萄糖6 - 磷酸在D2O中依次暴露于磷酸葡萄糖异构酶、磷酸果糖激酶和果糖 - 1,6 - 二磷酸酶产生，与当氘代酮己糖磷酸由D - 甘露糖6 - 磷酸在D2O中依次暴露于磷酸甘露糖异构酶、磷酸果糖激酶和果糖 - 1,6 - 二磷酸酶产生时记录的谱不同。通过1H NMR光谱鉴定D - 果糖6 - 磷酸的两种同位素异构体，为分别评估在磷酸葡萄糖异构酶和磷酸甘露糖异构酶催化的反应中己糖磷酸相互转化的相对程度提供了一种新工具。