Donatelli I, Castrucci M R, Campitelli L, Ruggieri A, Sidoli L, Buonavoglia C
Laboratory of Virology, Istituto Superiore di Sanita, Rome, Italy.
Comp Immunol Microbiol Infect Dis. 1991;14(4):315-23. doi: 10.1016/0147-9571(91)90137-3.
In Italy epizootics of equine influenza often occur, but no virus isolation has been reported since 1971. This paper describes the antigenic and biochemical characterization of two equine influenza viruses isolated in Italy from 1985 to 1989. The virus isolates were shown to differ antigenically from earlier strains of the same subtype, A/equine/Miami/1/63 (H3N8). Monoclonal antibody analysis showed that the haemagglutinins of these strains were serologically indistinguishable from A/equine/Fontainebleau/1/79, a variant of A/equine/Miami, never isolated in Italy before. One of the two virus isolates was obtained from a horse immunized with a bivalent inactivated influenza vaccine, not containing A/equine/Fontainebleau/79 antigens. The vaccine failure underlines the importance of antigenic relatedness between currently circulating viruses and vaccine strains. Therefore, to improve the protection afforded by equine immunization, the vaccine composition should be decided according to the results of a virological surveillance activity, systematically conducted among horses.
在意大利，马流感 epizootics 经常发生，但自1971年以来尚无病毒分离的报道。本文描述了1985年至1989年在意大利分离的两种马流感病毒的抗原和生化特性。这些病毒分离株在抗原性上与同一亚型的早期毒株A/equine/Miami/1/63（H3N8）不同。单克隆抗体分析表明，这些毒株的血凝素在血清学上与A/equine/Fontainebleau/1/79无法区分，A/equine/Fontainebleau/1/79是A/equine/Miami的一个变种，此前从未在意大利分离到。两种病毒分离株中的一种是从一匹用不含A/equine/Fontainebleau/79抗原的二价灭活流感疫苗免疫的马身上获得的。疫苗失效凸显了当前流行病毒与疫苗毒株之间抗原相关性的重要性。因此，为了提高马免疫提供的保护，应根据在马群中系统开展的病毒学监测活动的结果来确定疫苗组成。
