Wintermeyer Philip, Gerner Patrick, Gehring Stephan, Karimi Afshin, Wirth Stefan
Children's Hospital, Helios Klinikum Wuppertal, Witten-Herdecke University, Germany.
World J Gastroenterol. 2006 Apr 14;12(14):2235-8. doi: 10.3748/wjg.v12.i14.2235.
To find out whether there is a significant difference in the prevalence of the precore stop codon mutation between HBeAg positive and anti-HBe positive children.
We investigated a large pediatric population of 155 European children (mean age 10.9 years) with chronic hepatitis B by PCR and direct sequencing. Ninety were HBeAg positive and 65 had seroconversion to anti-HBe. Additionally genotyping was performed.
Seventy-four (48%) of the sequenced HBV strains were attributed to genotype D and 81 (52%) to genotype A. In the group of 90 HBeAg positive patients, 2 (2.2%) 1896-G-to-A transitions leading to precore stop codon mutation were found, and in the group of 65 anti-HBe positive children, 5 (7.7%) were identified harbouring HBeAg-minus mutants. The difference was not statistically significant (P = 0.13).
HBeAg minus variants as predominant viral HB strains play a minor role in the course of chronic hepatitis B in European children.
探究HBeAg阳性和抗-HBe阳性儿童中前核心区终止密码子突变的流行率是否存在显著差异。
我们通过PCR和直接测序对155名欧洲慢性乙型肝炎儿童（平均年龄10.9岁）的大样本人群进行了调查。其中90名儿童HBeAg阳性，65名已血清学转换为抗-HBe。此外，还进行了基因分型。
测序的HBV毒株中，74株（48%）属于D基因型，81株（52%）属于A基因型。在90名HBeAg阳性患者组中，发现2例（2.2%）发生了1896-G到A的转换，导致前核心区终止密码子突变；在65名抗-HBe阳性儿童组中，有5例（7.7%）被鉴定为携带HBeAg缺失突变体。差异无统计学意义（P = 0.13）。
在欧洲儿童慢性乙型肝炎病程中，作为主要病毒HB毒株的HBeAg缺失变异体起的作用较小。
