中药加味四逆散对乙型肝炎病毒转基因小鼠的抗病毒作用

Antiviral effect of Chinese medicine jiaweisinisan in hepatitis B virus transgenic mice.

作者信息

Chen Xiao-Yin, Tong Guang-Dong, Xia Fang

机构信息

Department of Traditional Chinese Medicine, Medical College, Jinan University, 601 Huangpu Road, Guangzhou 510632, Guangdong Province, China.

出版信息

World J Gastroenterol. 2006 Apr 14;12(14):2280-3. doi: 10.3748/wjg.v12.i14.2280.

DOI:10.3748/wjg.v12.i14.2280

PMID:16610037

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4087662/

Abstract

AIM

To study the antiviral effect of Chinese medicine jiaweisinisan (JWSNS) on hepatitis B virus (HBV) infection in transgenic mice (TGM).

METHODS

Twenty two 6-8 wk old HBV TGM in the third generation were divided into TGM control group and TGM treated group randomly. The normal control group included ten normal BC 57L/6 mice at the same age. The mice in treated group were administrated with JWSNS at the concentration of 4 g/mL and the dosage of 50 g/kg per d for 30 d, while the mice in TGM control group and normal control group were administrated with normal saline at the same dosage and the same time. Polymerase chain reaction (PCR) was used to assess the contents of HBV DNA in serum of HBV TGM before and after treatments, whereas blot hybridization was utilized to measure the contents of HBV DNA in the liver of both HBV TGM and normal BC 57L/6 mice.

RESULTS

The levels of serum HBV DNA in TGM treated group were remarkably decreased after the treatment of JWSNS (7.662+/-0.78 vs 5.22+/-3.14, P < 0.05), while there was no obvious change after administration of normal saline in TGM control group (7.125+/-4.26 vs 8.932+/-5.12, P > 0.05). The OD values of HBV DNA in the livers of the mice in TGM treated group were significantly lower than those of TGM control group (0.274+/-0.096 vs 0.432+/-0.119, P < 0.01).

CONCLUSION

JWSNS exerts suppressive effects on HBV DNA in the serum and liver of TGM.

摘要

目的

研究加味四逆散（JWSNS）对转基因小鼠（TGM）乙型肝炎病毒（HBV）感染的抗病毒作用。

方法

将22只6 - 8周龄的第三代HBV转基因小鼠随机分为转基因小鼠对照组和转基因小鼠治疗组。正常对照组包括10只同龄的正常C57L/6小鼠。治疗组小鼠以4 g/mL的浓度、50 g/kg的剂量每日给予加味四逆散，连续给药30天，而转基因小鼠对照组和正常对照组小鼠以相同剂量和相同时间给予生理盐水。采用聚合酶链反应（PCR）评估转基因小鼠治疗前后血清中HBV DNA的含量，同时利用印迹杂交法检测转基因小鼠和正常C57L/6小鼠肝脏中HBV DNA的含量。

结果

加味四逆散治疗后，转基因小鼠治疗组血清HBV DNA水平显著降低（7.662±0.78对5.22±3.14，P < 0.05），而转基因小鼠对照组给予生理盐水后无明显变化（7.125±4.26对8.932±5.12，P > 0.05）。转基因小鼠治疗组小鼠肝脏中HBV DNA的OD值显著低于转基因小鼠对照组（0.274±0.096对0.432±0.119，P < 0.01）。