Department of Infectious Disease, the Second Affiliated Hospital of Medical School of Xi'an, Jiao Tong University, Xi'an, Shannxi Province, China.
World J Gastroenterol. 2009 Dec 7;15(45):5669-73. doi: 10.3748/wjg.15.5669.
To evaluate the anti-viral effect of emodin plus Astragalus polysaccharide (APS) in hepatitis B virus (HBV) transgenic mice.
Sixty HBV transgenic mice (HBV TGM) whose weight varied between 18 and 24 g were randomly divided into 3 groups, with 20 mice in each group. Group A was the normal control, where the mice were treated with physiological saline; group B was the positive control where the mice were treated with lamivudine solution (100 mL/kg per day). Group C was the experimental group where the mice were treated with physiological saline containing emodin and APS (57.59 mg/kg per day and 287.95 mg/kg per day, respectively). The mice were treated daily for 3 wk. After 1 wk recovery time, the mice were sacrificed and serum as well as liver tissues were collected for ELISA and histological examination.
After 21 d treatment, HBV DNA levels in group B and group C significantly declined when compared with group A (P < 0.05). However, a significant increase in HBV DNA content was observed in group B, whereas this phenomenon was not observed in group C. A reduction in the contents of HBsAg, HBeAg and HBcAg in the mice from group B and C was observed when compared with group A.
Emodin and APS have a weak but persistent inhibitory effect on HBV replication in vivo, which may function as a supplementary modality in the treatment of hepatitis B infection.
评价大黄素联合黄芪多糖（APS）对乙型肝炎病毒（HBV）转基因小鼠的抗病毒作用。
将 60 只体重 18～24 g 的 HBV 转基因小鼠（HBV TGM）随机分为 3 组，每组 20 只。A 组为正常对照组，给予生理盐水；B 组为阳性对照组，给予拉米夫定溶液（100 mL/kg 每天）；C 组为实验组，给予含大黄素和 APS 的生理盐水（57.59 mg/kg 每天和 287.95 mg/kg 每天）。每天治疗 3 周。1 周恢复期后，处死小鼠，收集血清和肝组织，用于 ELISA 和组织学检查。
治疗 21 d 后，B 组和 C 组 HBV DNA 水平明显低于 A 组（P＜0.05）。但 B 组 HBV DNA 含量明显增加，而 C 组未见此现象。与 A 组相比，B 组和 C 组小鼠的 HBsAg、HBeAg 和 HBcAg 含量均降低。
大黄素和 APS 对 HBV 在体内的复制具有较弱但持续的抑制作用，可能作为乙型肝炎感染治疗的辅助手段。
