Ståhl S R
Department of Microbiology, University of Lund, Sweden.
Plasmid. 1991 Sep;26(2):94-107. doi: 10.1016/0147-619x(91)90049-3.
Obligately thermophilic strains of Bacillus stearothermophilus were screened for the presence of plasmids by agarose gel electrophoresis. All strains in our collection contained large plasmids (20 x 10(6)-80 x 10(6)) and were divided into four groups with respect to their plasmid pattern and production of bacteriocins. The major plasmid species were designated pSE407 (38.7 x 10(6)), pSE409 (29.0 x 10(6)), pSE411 (21.5 x 10(6)), and pSE410 (23.5 x 10(6)). Their physical endonuclease maps were constructed, and by Southern blots and hybridizations it was shown that these plasmids were related. From curing experiments and electrotransformations (electroporations) we conclude that pSE407, pSE410, and pSE411 code for temperature resistance. In addition pSE410 codes for bacteriocin production and resistance. Plasmid pSE409 probably also codes for bacteriocin production and resistance.
通过琼脂糖凝胶电泳筛选嗜热脂肪芽孢杆菌的嗜热菌株以检测质粒的存在。我们收集的所有菌株都含有大质粒（20×10⁶ - 80×10⁶），并根据其质粒图谱和细菌素的产生分为四组。主要的质粒种类被命名为pSE407（38.7×10⁶）、pSE409（29.0×10⁶）、pSE411（21.5×10⁶）和pSE410（23.5×10⁶）。构建了它们的物理内切酶图谱，通过Southern印迹和杂交表明这些质粒是相关的。从消除实验和电转化（电穿孔）中我们得出结论，pSE407、pSE410和pSE411编码耐热性。此外，pSE410编码细菌素的产生和抗性。质粒pSE409可能也编码细菌素的产生和抗性。
