Singab Abdel Nasser B, Ahmed Amer H, Sinkkonen Jari, Ovcharenko Vladimir, Pihlaja Kalevi
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo, Egypt.
Z Naturforsch C J Biosci. 2006 Jan-Feb;61(1-2):57-63. doi: 10.1515/znc-2006-1-211.
The molluscicidal activity of leaf and rhizome extracts of Iris germanica L. (var. alba) against Biomphalaria alexandrina snails was evaluated and the rhizome extracts were found to be the most potent. Activity-guided fractionation revealed that the chloroform extract showed the highest molluscicidal activity (LC90 = 1.26 mg/l) among the tested extracts of the rhizomes. Fraction B prepared from the chloroform extract was the most potent molluscicide (LC90 = 0.96 mg/l) in addition, it showed a significant heart rate reduction in the snail after a 6- to 24-h exposure period. It also displayed a significant level of cercaricidal potential in a time-concentration relationship pattern. Chromatographic fractionation and purification of fraction B resulted in the isolation of two novel compounds: 5,2'-dihydroxy-3-methoxy-6,7-methylenedioxyflavone and 5,7,2'-trihydroxy-6-methoxyflavanone. Their structures were established by one- and two-dimensional NMR methods and mass spectrometry.
对德国鸢尾（白变种）叶片和根茎提取物对亚历山大双脐螺的杀螺活性进行了评估，发现根茎提取物的活性最强。活性导向分离表明，在测试的根茎提取物中，氯仿提取物表现出最高的杀螺活性（LC90 = 1.26毫克/升）。从氯仿提取物制备的组分B是最有效的杀螺剂（LC90 = 0.96毫克/升），此外，在6至24小时的暴露期后，它使蜗牛的心率显著降低。它还在时间-浓度关系模式中显示出显著的杀尾蚴潜力。对组分B进行色谱分离和纯化，得到了两种新化合物：5,2'-二羟基-3-甲氧基-6,7-亚甲二氧基黄酮和5,7,2'-三羟基-6-甲氧基黄烷酮。通过一维和二维核磁共振方法以及质谱确定了它们的结构。
