Doungchawee Galayanee, Phulsuksombat Duangporn, Naigowit Pimjai, Khoaprasert Yuvaluk, Sangjun Noppadon, Kongtim Suraphol, Smythe Lee
Department of Pathobiology, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewee, Bangkok 10400, Thailand.
Southeast Asian J Trop Med Public Health. 2005 Nov;36(6):1516-22.
During 1999-2000, kidney tissues of approximately 15% of 1310 rodents trapped from northeastern provinces of Thailand were tested for the presence of leptospires. Our direct immunofluorescent assay (DFA) for detection of leptospires showed 100% sensitivity and 94% specificity with the culture data. Both methods identified R. norvegicus as the highest source of infection. Among isolated Leptospira, 137 were serotyped by cross agglutinin absorption and/or a microscopic agglutination, and gave some variations and similarities at the serovar level to the DFA results. DFA data demonstrated over half of the positive animals were infected with several serovars of Leptospira interrogans. A subsequent DFA study in Bangkok in 2002 revealed leptospiral infection in 33% of 42 rats and shrews. The most common infecting serovars were Autumnalis and Canicola identified in rural and urban animals, respectively. This finding suggests that wild small mammals may act as important sources of pathogenic leptospires and warrant active surveillance to understand the epidemiology of transmission and control of carrier animals.
在1999年至2000年期间，对从泰国东北部省份捕获的1310只啮齿动物中约15%的肾脏组织进行了钩端螺旋体检测。我们用于检测钩端螺旋体的直接免疫荧光测定法（DFA）与培养数据相比，灵敏度为100%，特异性为94%。两种方法均确定褐家鼠是最高感染源。在分离出的钩端螺旋体中，137株通过交叉凝集素吸收和/或显微镜凝集进行血清分型，在血清型水平上与DFA结果存在一些差异和相似之处。DFA数据表明，超过一半的阳性动物感染了几种问号钩端螺旋体血清型。2002年在曼谷进行的一项后续DFA研究显示，在42只大鼠和鼩鼱中有33%感染了钩端螺旋体。最常见的感染血清型分别是在农村和城市动物中发现的秋季热群和犬群。这一发现表明，野生小型哺乳动物可能是致病性钩端螺旋体的重要来源，需要进行积极监测以了解传播的流行病学情况并控制携带动物。
