Agudelo-Flórez Piedad, Londoño Andrés F, Quiroz Víctor H, Angel Juan C, Moreno Natalí, Loaiza Erica T, Muñoz Luis F, Rodas Juan D
Instituto Colombiano de Medicina Tropical-Universidad CES, Colombia.
Am J Trop Med Hyg. 2009 Nov;81(5):906-10. doi: 10.4269/ajtmh.2009.09-0195.
Leptospirosis is a widely distributed zoonosis, and rats are its most common source of infection. Our goal was to determine the frequency for Leptospira infection in rodents in a farmers market in the city of Medellin. We performed a descriptive transversal study sampling 254 rodents. Rodents were bled and killed, and kidneys samples were taken. Supernatants of macerated kidneys were cultured on Fletcher medium. Microagglutination tests (MATs) with 11 serovars were also carried out in rat serum, and a polymerase chain reaction (PCR) specific for pathogenic species was used to test each bacterial culture. All animals were identified as Rattus norvegicus; 25% and 20% were positive by MAT and culture, respectively. PCR tests of 12 isolates were positive for pathogenic serovars, and 4 of them were confirmed as L. interrogans by sequencing. These data show the role of this natural carrier and shedder of pathogenic leptospires in the epidemiology of urban leptospirosis in Colombia.
钩端螺旋体病是一种广泛分布的人畜共患病，鼠类是其最常见的感染源。我们的目标是确定麦德林市一个农贸市场啮齿动物中钩端螺旋体感染的频率。我们进行了一项描述性横断面研究，对254只啮齿动物进行采样。采集啮齿动物的血液并将其处死，然后取肾脏样本。将研磨后的肾脏上清液接种于弗莱彻培养基上培养。还对大鼠血清进行了针对11个血清型的显微凝集试验（MAT），并使用针对致病菌种的聚合酶链反应（PCR）检测每个细菌培养物。所有动物均鉴定为褐家鼠；MAT和培养法检测的阳性率分别为25%和20%。对12株分离株进行的PCR检测显示致病血清型呈阳性，其中4株经测序确认为问号钩端螺旋体。这些数据表明了这种致病性钩端螺旋体的天然携带者和排出者在哥伦比亚城市钩端螺旋体病流行病学中的作用。
