Pallardó Federico V, Degan Paolo, d'Ischia Marco, Kelly Frank J, Zatterale Adriana, Calzone Rita, Castello Giuseppe, Fernandez-Delgado Rafael, Dunster Christina, Lloret Ana, Manini Paola, Pisanti Maria Antonietta, Vuttariello Emilia, Pagano Giovanni
Department of Physiology, University of Valencia, E-46010, Valencia, Spain.
Biogerontology. 2006 Aug;7(4):211-20. doi: 10.1007/s10522-006-9002-5. Epub 2006 Apr 13.
Oxidative stress has been associated with Down syndrome (DS) and with its major phenotypic features, such as early ageing. In order to evaluate an in vivo pro-oxidant state, the following analytes were measured in a group of DS patients aged 2 months to 57 years: (a) leukocyte 8-hydroxy-2'-deoxyguanosine (8-OHdG); (b) blood glutathione; (c) plasma levels of: glyoxal (Glx) and methylglyoxal (MGlx); some antioxidants (uric acid, UA, ascorbic acid, AA and Vitamin E), and xanthine oxidase (XO) activity. A significant 1.5-fold increase in 8-OHdG levels was observed in 28 DS patients vs. 63 controls, with a sharper increase in DS patients aged up to 30 years. The GSSG:GSH x 100 ratio was significantly higher in young DS patients (< 15 years), in contrast to DS patients aged >or=15 years that showed a significant decrease in the GSSG:GSH x 100 ratio ratio vs. controls of the respective age groups. Plasma Glx levels were significantly higher in young DS patients, whereas no significant difference was detected in DS patients aged >or=15 years. Unlike Glx, the plasma levels of MGlx were found to be significantly lower in DS patients vs. controls. A significant increase was observed in plasma levels of UA in DS patients that could be related to an increased plasma XO activity in DS patients. The plasma concentrations of AA were also increased in young (< 15 years) DS patients, but not in older patients vs. controls in the same age range. The levels of Vitamin E in DS patients did not differ from the values determined in control donors. The evidence for a multiple pro-oxidant state in young DS patients supports the role of oxidative stress in DS phenotype, with relevant distinctions according to patients' ages.
氧化应激与唐氏综合征（DS）及其主要表型特征（如早衰）有关。为了评估体内的促氧化状态，对一组年龄在2个月至57岁的DS患者进行了以下分析物的测量：（a）白细胞8-羟基-2'-脱氧鸟苷（8-OHdG）；（b）血液中的谷胱甘肽；（c）血浆水平：乙二醛（Glx）和甲基乙二醛（MGlx）；一些抗氧化剂（尿酸，UA，抗坏血酸，AA和维生素E），以及黄嘌呤氧化酶（XO）活性。与63名对照组相比，28名DS患者的8-OHdG水平显著升高了1.5倍，在30岁及以下的DS患者中升高更为明显。年轻DS患者（<15岁）的GSSG:GSH×100比值显著更高，而年龄≥15岁的DS患者与相应年龄组的对照组相比，GSSG:GSH×100比值显著降低。年轻DS患者的血浆Glx水平显著更高，而年龄≥15岁的DS患者未检测到显著差异。与Glx不同，DS患者的血浆MGlx水平显著低于对照组。DS患者的血浆UA水平显著升高，这可能与DS患者血浆XO活性增加有关。年轻（<15岁）DS患者的血浆AA浓度也升高，但与同年龄范围的老年患者相比，与对照组相比未升高。DS患者的维生素E水平与对照供体中测定的值没有差异。年轻DS患者存在多种促氧化状态的证据支持氧化应激在DS表型中的作用，且根据患者年龄存在相关差异。
