环磷酸腺苷（cAMP）水平升高可增强人骨髓单核细胞系和单核细胞中白细胞介素-1α（IL-1α）及白细胞介素-1β（IL-1β）的信使核糖核酸（mRNA）表达和蛋白质生成。

Increased cyclic AMP levels enhance IL-1 alpha and IL-1 beta mRNA expression and protein production in human myelomonocytic cell lines and monocytes.

作者信息

Sung S S, Walters J A

机构信息

Department of Radiation Oncology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.

出版信息

J Clin Invest. 1991 Dec;88(6):1915-23. doi: 10.1172/JCI115515.

The effects of increasing intracellular cAMP levels on IL-1 alpha and IL-1 beta mRNA expression and IL-1 production in human monocytes and nonlymphoid hematopoietic cell lines were examined. Peripheral monocytes and myelomonocytic cell lines could be stimulated by LPS or phorbol myristate acetate (PMA) to express IL-1 mRNA. Dibutyryl cAMP, 8-bromo-cAMP, forskolin, cholera toxin, PGE1, and PGE2 synergized with PMA or LPS to increase the accumulation in cell lines of IL-1 alpha mRNA by up to 50-fold and that of IL-1 beta mRNA by 10- to 20-fold compared to LPS or PMA alone. This increase in IL-1 alpha and IL-1 beta mRNA accumulation was more modest in monocytes. The synergistic stimulation was due to enhanced IL-1 gene transcription rate rather than increased IL-1 mRNA stability. Despite this marked increase in IL-1 mRNA accumulation, IL-1 protein synthesis in these cells was increased by only twofold. Thus, IL-1 synthesis in monocytes and myelomonocytic cell lines is under stringent translational control.

研究了细胞内cAMP水平升高对人单核细胞和非淋巴细胞造血细胞系中IL-1α和IL-1β mRNA表达及IL-1产生的影响。外周单核细胞和骨髓单核细胞系可被脂多糖（LPS）或佛波酯（PMA）刺激以表达IL-1 mRNA。与单独使用LPS或PMA相比，二丁酰cAMP、8-溴-cAMP、福斯可林、霍乱毒素、前列腺素E1（PGE1）和前列腺素E2（PGE2）与PMA或LPS协同作用，使细胞系中IL-1α mRNA的积累增加高达50倍，IL-1β mRNA的积累增加10至20倍。单核细胞中IL-1α和IL-1β mRNA积累的这种增加较为适度。这种协同刺激是由于IL-1基因转录速率增强而非IL-1 mRNA稳定性增加所致。尽管IL-1 mRNA积累显著增加，但这些细胞中IL-1蛋白合成仅增加两倍。因此，单核细胞和骨髓单核细胞系中的IL-1合成受到严格的翻译控制。