两种不同的人白细胞介素-1互补DNA的克隆、测序及表达
Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs.
作者信息
March C J, Mosley B, Larsen A, Cerretti D P, Braedt G, Price V, Gillis S, Henney C S, Kronheim S R, Grabstein K
出版信息
Nature. 1985;315(6021):641-7. doi: 10.1038/315641a0.
DOI:10.1038/315641a0
PMID:2989698
Abstract
Two distinct but distantly related complementary DNAs encoding proteins sharing human interleukin-1 (IL-1) activity (termed IL-1 alpha and IL-1 beta), were isolated from a macrophage cDNA library. The primary translation products of the genes are 271 and 269 amino acids long, although expression in Escherichia coli of the carboxy-terminal 159 and 153 amino acids produces IL-1 biological activity.
摘要
从巨噬细胞cDNA文库中分离出了两个不同但关系较远的互补DNA，它们编码的蛋白质具有人白细胞介素-1（IL-1）活性（分别称为IL-1α和IL-1β）。这些基因的初级翻译产物分别长271和269个氨基酸，不过在大肠杆菌中表达羧基末端的159和153个氨基酸时可产生IL-1生物活性。
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