[核因子-κB mRNA及蛋白在胃溃疡复发大鼠胃组织中的表达及健胃愈疡颗粒对其表达的影响]

[Nuclear factor-kappaB mRNA and protein expression in stomach tissue of rats with gastric ulcer recurrence and effect of jianwei yuyang granule on its expression].

作者信息

Ling Jiang-Hong, Li Jia-Bang, Shen Ding-Zhu, Zhou Bing

机构信息

Institute of Integrated Traditional Chinese and West Medicine, Xiangya Hospital , Zhongnan University, Changsha (410008).

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006 Mar;26(3):228-31.

PMID:16613268

Abstract

OBJECTIVE

To observe the inflammatory reaction, nuclear factor-kappaB (NF-kappaB) mRNA and protein expression in stomach tissue of rats with gastric ulcer recurrence and the effect of Jianwei Yuyang granule (JYG) on them.

METHODS

Gastric ulcer and its recurrent lesion were successively induced by acetic acid and interliukin1-beta (IL-1beta), and the model rats were divided into the sham operation group, the model group, the omeprazole (correction of omepraxole) group and the JYG group to observe the state of chronic inflammatory cell, neutrophil count, NF-kappaBmRNA and protein expression in stomach tissue.

RESULTS

On the 16th and 92th day after administration, the increase of chronic inflammatory cell, neutrophil, NF-kappaBmRNA and protein expression in the model group was more significant than those in the sham operated group (P < 0.01), while that was lower in the JYG group than in the model group (P < 0.05, P <0.01), but with no remarkable difference to the omepraxole group. In addition, the mRNA and protein expression of NF-kappaB were correlated closely with the count of chronic inflammatory cell and neutrophil respectively (P < 0.01).

CONCLUSION

NF-kappaB may play an important role in regulating inflammatory reaction during the healing and recurrence processes of gastric ulcer induced by acetic acid. JYG may suppress inflammatory reaction by inhibiting the activation and expression of NF-kappaB in stomach tissue, which may be one of the mechanisms of JYG in preventing the recurrence of gastric ulcer.

摘要

目的

观察胃溃疡复发大鼠胃组织的炎症反应、核因子-κB（NF-κB）mRNA及蛋白表达情况，以及健胃愈疡颗粒（JYG）对其的影响。

方法

先后用乙酸和白细胞介素1-β（IL-1β）诱导大鼠胃溃疡及其复发病变，将造模大鼠分为假手术组、模型组、奥美拉唑组和JYG组，观察胃组织中慢性炎症细胞状态、中性粒细胞计数、NF-κB mRNA及蛋白表达情况。

结果

给药后第16天和第92天，模型组慢性炎症细胞、中性粒细胞、NF-κB mRNA及蛋白表达的升高幅度均显著高于假手术组（P<0.01），而JYG组低于模型组（P<0.05，P<0.01），但与奥美拉唑组无显著差异。此外，NF-κB的mRNA和蛋白表达分别与慢性炎症细胞计数和中性粒细胞计数密切相关（P<0.01）。