Yu Yan, Jia Tian-Zhu, Cai Qian
Experimental Center of Traditional Chinese Medicine, the Affiliated Hospital, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
Zhongguo Zhong Yao Za Zhi. 2016 Feb;41(4):705-710. doi: 10.4268/cjcmm20160427.
To compare the anti-inflammatory activity of the crude Atractylodes lancea (AL) and AL processed products by stir-baking with bran in rat models of gastric ulcer, and preliminarily explore the anti-ulcer mechanisms of AL, the model of gastric ulcer was imitated by local acetic acid injection into gastric mucosa in rats by surgery according to the modified Okabe method. All rats were randomly divided into the following 10 groups： sham-operation group, model group, omeprazole group, Sanjiu Weitai granule group, crude AL low dose group, crude AL middle dose group, crude AL high dose group, processed AL low dose group, processed AL middle dose group, and processed AL high dose group. Rats were administered via intragastric (ig) two times each day, for 10 consecutive days. Blood was collected from the abdominal aorta, serum was separated, and the ulcer tissues were taken. The levels of inflammatory factors interleukin 6, 8 (IL-6, 8), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2) in serum and gastric tissues were determined by enzyme-linked immunosorbent assay (ELISA), and the mRNA expressions of TNF-α and IL-8 in gastric tissues were detected by quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). The protein expressions of TNF-α and IL-8 in gastric tissues were detected by immunohistochemistry. Compared with sham-operation group, the levels of TNF-α, IL-8, IL-6, PGE2 as well as the mRNA expressions and protein expressions of TNF-α, IL-8 in gastric tissues were significantly higher in model group. The above levels were reduced in different degrees in all treatment groups. Compared with the crude AL, same dose of processed AL was more effective in decreasing the levels of TNF-α, IL-8, IL-6, PGE2 in serum and gastric tissues and down-regulating the mRNA expressions of TNF-α and IL-8 in gastric tissues, with significant difference in middle dose groups and high dose groups. The results showed that AL had potent anti-inflammatory effects in rat models of gastric ulcer induced by acetic acid, and the processed AL had more obvious effect. The anti-ulcer action of AL could be attributed partly to down-regulating the levels of TNF-α, IL-8, IL-6 and PGE2.
为比较生苍术（AL）及其麸炒炮制品在大鼠胃溃疡模型中的抗炎活性，并初步探讨苍术的抗溃疡机制，按照改良冈部法通过手术向大鼠胃黏膜局部注射乙酸来模拟胃溃疡模型。将所有大鼠随机分为以下10组：假手术组、模型组、奥美拉唑组、三九胃泰颗粒组、生苍术低剂量组、生苍术中剂量组、生苍术高剂量组、炮制品苍术低剂量组、炮制品苍术中剂量组、炮制品苍术高剂量组。大鼠每天经胃内（ig）给药2次，连续给药10天。从腹主动脉采集血液，分离血清，并取溃疡组织。采用酶联免疫吸附测定（ELISA）法测定血清和胃组织中炎症因子白细胞介素6、8（IL-6、8）、肿瘤坏死因子-α（TNF-α）和前列腺素E2（PGE2）的水平，采用定量实时逆转录聚合酶链反应（RT-PCR）法检测胃组织中TNF-α和IL-8的mRNA表达。采用免疫组织化学法检测胃组织中TNF-α和IL-8的蛋白表达。与假手术组相比，模型组胃组织中TNF-α、IL-8、IL-6、PGE2水平以及TNF-α、IL-8的mRNA表达和蛋白表达均显著升高。所有治疗组上述水平均有不同程度降低。与生苍术相比，相同剂量的炮制品苍术在降低血清和胃组织中TNF-α、IL-8、IL-6、PGE2水平以及下调胃组织中TNF-α和IL-8的mRNA表达方面更有效，中剂量组和高剂量组有显著差异。结果表明，苍术在乙酸诱导的大鼠胃溃疡模型中具有较强的抗炎作用，且炮制品苍术的作用更明显。苍术的抗溃疡作用可能部分归因于下调TNF-α、IL-8、IL-6和PGE2的水平。
