Ding Lai-Biao, Lu Fu-Er, Ye Ai-Li, Xu Lijun, Wang Kaifu
Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital Affilliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006 Mar;26(3):232-5.
To study effects of Huanglian Jiedu Decoction (HJD) on lipid metabolism, leptin and resistin in insulin resistive rats.
Type 2 diabetes mellitus (DM2) rat model was induced by intravenous injection with a low dose of streptozotocin and high fat and high caloric diet, and randomized into four groups: the model control group, the HJD group, the berberine group and the aspirin group. Meanwhile 16 normal rats were assigned as the normal control group. The body weight was observed before and after treatment, total cholesterol (TC), triglycerides (TG), high density lipoprotein-cholesterol (HDL-C), apolipoprotein A1 and B (apoA1, apoB), free fatty acid (FFA), insulin (INS) in serum, skeletal muscle triglyceride (mTG) in skeletal muscle, and oral glucose tolerance test (OGTT) and fasting blood glucose (FBG) were detected after 10 weeks of treatment, at the same time, serum levels of leptin and resistin were determined using ELLISA.
The levels of TC, TG, apoB, FBG, FFA, mTG, leptin and resistin were obviously lowered, the levels of HDL-C and apoA1 significantly raised, glucose tolerance improved and body weight reduced in insulin resistive rats, as compared with the model control (P<0.05, P<0.01).
The effects of HJD in lowering lipids, as well as reducing leptin and resistin might be closely correlated with its effects in ameliorating insulin resistance.
研究黄连解毒汤对胰岛素抵抗大鼠脂质代谢、瘦素和抵抗素的影响。
采用小剂量链脲佐菌素腹腔注射结合高脂高糖饮食诱导2型糖尿病大鼠模型，随机分为四组：模型对照组、黄连解毒汤组、黄连素组和阿司匹林组。同时选取16只正常大鼠作为正常对照组。观察治疗前后体重变化，治疗10周后检测血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、载脂蛋白A1和B（apoA1、apoB）、游离脂肪酸（FFA）、胰岛素（INS）、骨骼肌甘油三酯（mTG），进行口服葡萄糖耐量试验（OGTT）及空腹血糖（FBG）检测，同时采用酶联免疫吸附测定法（ELLISA）检测血清瘦素和抵抗素水平。
与模型对照组比较，胰岛素抵抗大鼠的TC、TG、apoB、FBG、FFA、mTG、瘦素和抵抗素水平明显降低，HDL-C和apoA1水平显著升高，糖耐量改善，体重减轻（P<0.05，P<0.01）。
黄连解毒汤降血脂、降低瘦素和抵抗素的作用可能与其改善胰岛素抵抗的作用密切相关。
