Dong Hiep Phuc, Elstrand Mari Bunkholt, Holth Arild, Silins Ilvars, Berner Aasmund, Trope Claes G, Davidson Ben, Risberg Björn
Department of Pathology, Norwegian Radium Hospital, University of Oslo, Norway.
Am J Clin Pathol. 2006 Mar;125(3):451-8.
We studied the clinical role of leukocyte infiltration and chemokine receptor expression in ovarian carcinoma effusions. Expression of leukocyte markers (CD3, CD4, CD8, CD4/CD8 ratio, CD16, CD19, and CD14) and chemokine receptors (CXCR1, CXCR4, CCR2, CCR5, and CCR7) was studied in 73 effusions by using flow cytometry. CXCR4, CCR5, and CCR7 were expressed abundantly on leukocytes, but all receptors were expressed rarely on cancer cells. The presence of natural killer cells (P = .042) and International Federation of Gynecology and Obstetrics (FIGO) stage IV disease (P = .024) predicted worse overall survival (OS). A higher percentage of CD19+ cells (P = .015) and stage IV disease (P = .008) predicted poor survival for patients with postchemotherapy effusions. Only FIGO stage retained significance as a predictor of OS (P = .035) in multivariate analysis. Chemokine receptors are expressed widely on leukocytes but rarely on carcinoma cells in ovarian carcinoma effusions, arguing against an autocrine chemokine pathway in this malignancy. Immune response parameters in ovarian cancer effusions are weak predictors of outcome.
我们研究了白细胞浸润和趋化因子受体表达在卵巢癌腹腔积液中的临床作用。通过流式细胞术研究了73例腹腔积液中白细胞标志物（CD3、CD4、CD8、CD4/CD8比值、CD16、CD19和CD14）和趋化因子受体（CXCR1、CXCR4、CCR2、CCR5和CCR7）的表达。CXCR4、CCR5和CCR7在白细胞上大量表达，但所有受体在癌细胞上均很少表达。自然杀伤细胞的存在（P = 0.042）和国际妇产科联盟（FIGO）IV期疾病（P = 0.024）预示着总生存期（OS）较差。较高比例的CD19+细胞（P = 0.015）和IV期疾病（P = 0.008）预示着化疗后腹腔积液患者的生存期较差。在多变量分析中，只有FIGO分期作为OS的预测指标仍具有显著性（P = 0.035）。趋化因子受体在卵巢癌腹腔积液的白细胞上广泛表达，但在癌细胞上很少表达，这表明在这种恶性肿瘤中不存在自分泌趋化因子途径。卵巢癌腹腔积液中的免疫反应参数对预后的预测作用较弱。
