在大鼠哮喘模型中小气道和肺泡中水通道蛋白 1 和水通道蛋白 5 的变化。

Changes in water channel aquaporin 1 and aquaporin 5 in the small airways and the alveoli in a rat asthma model.

机构信息

Department of Histology and Embryology, Basic Medical College, Xinjiang Medical University, Urumqi 830011, China.

出版信息

Micron. 2013 Feb;45:68-73. doi: 10.1016/j.micron.2012.10.016. Epub 2012 Nov 10.

DOI:10.1016/j.micron.2012.10.016

PMID:23199524

Abstract

OBJECTIVES

To examine changes in aquaporin 1 (AQP1) and aquaporin 5 (AQP5) in the small airways and alveoli in a rat asthma model.

METHOD

Forty Wistar rats were randomly divided into a control group and an ovalbumin (OVA) sensitization asthma model group. The distribution and expression of AQP1 and AQP5 in lung tissues were analyzed using immunohistochemistry (IHC), quantified the staining intensity by assessing integrated optical densities (IOD), and Western blotting (WB).

RESULTS

IHC showed AQP1 was mainly distributed in sub-epithelial microvascular endothelial cells (MECs) and red blood cells. IOD values showed, in the asthma model group, the expression of AQP1 in alveolar MECs was lower than that in the control group (P<0.05); However, AQP1 expression in small airways sub-epithelial was higher than in the control group (P<0.05). The WB indicated that AQP1 expression in the asthma model group was 57% lower than in the control group (P<0.05). AQP5 was mainly distributed in the non-ciliated epithelial cells of the small airways and the apical membranes of type I and type II epithelial cells. IOD values showed, in asthma model group, the expression of AQP5 increased in small airways epithelium (P<0.05), and decreased in alveolar epithelium (P<0.05). The WB showed a 36% reduction in AQP5 expression compared with the control group (P<0.05).

CONCLUSION

AQP1 and AQP5 increased in small airways in rats with experimentally induced asthma, indicating that they may be involved in the formation of submucosal edema and mucus hypersecretion. Decreased AQP1 and AQP5 in pulmonary alveoli may be related to increased alveolar liquid viscosity and the formation of mucus plugs.

摘要

目的

检测水通道蛋白 1（AQP1）和水通道蛋白 5（AQP5）在哮喘大鼠模型中小气道和肺泡中的变化。

方法

40 只 Wistar 大鼠随机分为对照组和卵清蛋白（OVA）致敏哮喘模型组。采用免疫组织化学（IHC）分析肺组织中 AQP1 和 AQP5 的分布和表达，通过评估整合光密度（IOD）量化染色强度，并采用 Western blot（WB）进行检测。

结果

免疫组化显示 AQP1 主要分布于上皮下微脉管内皮细胞（MEC）和红细胞中。IOD 值显示，在哮喘模型组中，肺泡 MEC 中的 AQP1 表达低于对照组（P<0.05）；然而，小气道上皮下的 AQP1 表达高于对照组（P<0.05）。WB 结果表明，哮喘模型组 AQP1 表达水平较对照组降低了 57%（P<0.05）。AQP5 主要分布于小气道无纤毛上皮细胞和 I 型和 II 型上皮细胞的顶膜。IOD 值显示，在哮喘模型组中，小气道上皮中 AQP5 的表达增加（P<0.05），肺泡上皮中 AQP5 的表达减少（P<0.05）。WB 结果显示，与对照组相比，AQP5 表达降低了 36%（P<0.05）。