Xie J T, Wang C Z, Wicks S, Yin J J, Kong J, Li J, Li Y C, Yuan C S
Tang Center for Herbal Medicine Research, the Pritzker School of Medicine, University of Chicago, USA.
Exp Oncol. 2006 Mar;28(1):25-9.
Ganoderma lucidum is a commonly used Chinese herb and an important ingredient in traditional Chinese medicine herbal formulations for immune dysfunction related illnesses. The effects of this medicinal mushroom on human colorectal cancer cells have not yet been evaluated. In this study, we investigated the effects of Ganoderma lucidum extract using SW 480 human colorectal cancer cell line.
Two different fractions of Ganoderma lucidum extract, i.e., a fraction containing mainly polysaccharides (GLE-1), and a triterpenoid fraction without polysaccharides (GLE-2) were analyzed. Their antiproliferative activity was evaluated by cell proliferation assay and 3H-thymidine incorporation assay. Scavenging effects of DPPH radical were assessed using ESR-spectroscopy.
Our data showed that both GLE-1 and GLE-2 significantly inhibited the proliferation of SW 480 cells. The inhibitory effect of GLE-2 was much stronger than that of GLE-1. GLE-1 inhibited DNA synthesis in the cells and reduced the formation of DPPH radicals.
Ganoderma lucidum extract inhibits proliferation of human colorectal cancer cells and possesses antioxidant properties.
灵芝是一种常用的中药材，也是用于治疗免疫功能障碍相关疾病的中药配方中的重要成分。这种药用真菌对人结肠癌细胞的作用尚未得到评估。在本研究中，我们使用SW 480人结肠癌细胞系研究了灵芝提取物的作用。
分析了灵芝提取物的两种不同组分，即主要含多糖的组分（GLE-1）和不含多糖的三萜类组分（GLE-2）。通过细胞增殖试验和³H-胸腺嘧啶核苷掺入试验评估它们的抗增殖活性。使用电子自旋共振光谱法评估DPPH自由基的清除作用。
我们的数据表明，GLE-1和GLE-2均显著抑制SW 480细胞的增殖。GLE-2的抑制作用比GLE-1强得多。GLE-1抑制细胞中的DNA合成并减少DPPH自由基的形成。
灵芝提取物抑制人结肠癌细胞的增殖并具有抗氧化特性。
