Zhao M, Kim Y T, Yoon B S, Kim S W, Kang M H, Kim S H, Kim J H, Kim J W, Park Y W
Department of Obstetrics and Gynecology, Women's life and Science Institute, Yonsei University College of Medicine, Seoul, Korea.
Exp Oncol. 2006 Mar;28(1):44-8.
Cyclins are a family of regulatory proteins that play a key role in controlling the cell cycle. Abnormalities of cell cycle regulators, including cyclins and cyclin dependent kinases, have been reported in various malignant tumors. This study was undertaken to quantitatively detect cyclin B1 and D1 in cervical cancer.
A quantitative real-time reverse transcription polymerase chain reaction and Western blot assay were used to analyze the expression of cyclin B1/D1 mRNA and proteins, respectively, in fresh invasive cervical cancer (n = 41) and normal cervical tissues (n = 10).
There was significantly greater cyclin B1 expression in invasive cervical cancer than in normal cervical tissue (P = 0.019). However, cyclin D1 expression was not significantly different. A Western blot assay yielded similar results.
Our results were consistent with the concept that up-regulation of cyclin B1 expression occurred in cervical cancer and an aberrant expression of cyclin B1 might play an important role in cervical carcinogenesis.
细胞周期蛋白是一类调节蛋白，在控制细胞周期中起关键作用。包括细胞周期蛋白和细胞周期蛋白依赖性激酶在内的细胞周期调节因子异常已在各种恶性肿瘤中被报道。本研究旨在定量检测宫颈癌中细胞周期蛋白B1和D1。
采用定量实时逆转录聚合酶链反应和蛋白质免疫印迹法分别分析41例新鲜浸润性宫颈癌组织和10例正常宫颈组织中细胞周期蛋白B1/D1 mRNA和蛋白质的表达。
浸润性宫颈癌中细胞周期蛋白B1的表达显著高于正常宫颈组织（P = 0.019）。然而，细胞周期蛋白D1的表达无显著差异。蛋白质免疫印迹法得出了相似的结果。
我们的结果与宫颈癌中细胞周期蛋白B1表达上调的概念一致，且细胞周期蛋白B1的异常表达可能在宫颈癌发生中起重要作用。
