Gamaleia N F, Skivka L M, Fedorchuk A G, Shishko E D
Institute for Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Kyiv.
Exp Oncol. 2006 Mar;28(1):54-60.
To study circadian rhythms (CR) of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma were compared with those in healthy men.
The NK-cell and phagocyte cytotoxic activity in five patients with malignant melanoma stage I or II and 12 healthy donors has been assessed by radioimmune assay and NBT-test.
The circadian rhythmicity in NK-cells and phagocyte activity in all cancer patients under study has been disrupted. The extent of such disruption tended to increase in patients with more advanced cancer. The most typical alterations were discoordination between the cytotoxicity rhythms of NK-cells and phagocytes (synchronized in healthy persons) and alterations in basic rhythm parameters: phase shifts and amplitude damping.
In melanoma patients the significant alteration of CR in NK-cells and phagocytes cytotoxic activity was revealed. In spite of individual variations, the degree of the rhythm disruption basically depended on a disease stage. The alteration of CR phase and amplitude and discoordination between the rhythms of NK-cells and phagocyte were registered in all cases studied.
研究恶性黑色素瘤患者外周血单个核细胞细胞毒性活性的昼夜节律，并与健康男性进行比较。
通过放射免疫测定和NBT试验评估了5例Ⅰ期或Ⅱ期恶性黑色素瘤患者和12名健康供者的NK细胞和吞噬细胞细胞毒性活性。
所有研究的癌症患者中，NK细胞和吞噬细胞活性的昼夜节律均受到破坏。癌症进展程度越高的患者，这种破坏程度往往越大。最典型的改变是NK细胞和吞噬细胞的细胞毒性节律之间的失调（在健康人中是同步的）以及基本节律参数的改变：相位偏移和振幅衰减。
在黑色素瘤患者中，NK细胞和吞噬细胞细胞毒性活性的昼夜节律有显著改变。尽管存在个体差异，但节律破坏程度基本取决于疾病阶段。在所有研究病例中均记录到昼夜节律相位和振幅的改变以及NK细胞和吞噬细胞节律之间的失调。
