Masserini C, Villa M L, Rovini D, Clerici E
Boll Ist Sieroter Milan. 1981;60(4):307-15.
Thirty-three malignant melanoma patients were tested for NK cytotoxicity of peripheral blood lymphocytes in a Cr-release assay. NK activity was significantly lower in patients as compared with controls. In eight patients no cytotoxicity whatsoever could be detected. We tried to understand this NK impairment by using further assays such as the removal of plastic adherent cells or of carbonyl-iron phagocytizing cells and by mixing experiments. Only three patients showed the presence of NK activity suppressing cells and one patient had suppressive factors in his serum. No correlation was seen between NK activity values and clinical-biological parameters such as thickness of tumor, stage of disease, rate of recurrences or hystological findings.
采用铬释放试验检测了33例恶性黑色素瘤患者外周血淋巴细胞的自然杀伤细胞（NK）细胞毒性。与对照组相比，患者的NK活性显著降低。8例患者未检测到任何细胞毒性。我们通过进一步的试验，如去除塑料贴壁细胞或羰基铁吞噬细胞以及混合实验，试图了解这种NK功能受损的情况。只有3例患者显示存在NK活性抑制细胞，1例患者血清中有抑制因子。NK活性值与临床生物学参数，如肿瘤厚度、疾病分期、复发率或组织学结果之间未发现相关性。
