Barbosa Fernando, Corrêa Rodrigues Maria Heloísa, Buzalaf Maria R, Krug Francisco J, Gerlach Raquel F, Tanus-Santos José Eduardo
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ave. do Café s/n, Monte Alegre, 14049-903 Ribeirao Preto, SP, Brazil.
Arch Toxicol. 2006 Oct;80(10):633-7. doi: 10.1007/s00204-006-0096-y. Epub 2006 Apr 14.
We conducted a study to evaluate the use of parotid salivary lead (Pb-saliva) levels as a surrogate of the blood lead (Pb-B) or plasma lead levels (Pb-P) to diagnose lead exposure. The relationship between these biomarkers was assessed in a lead exposed population. Pb-saliva and Pb-P were determined by inductively coupled plasma mass spectrometry, while in whole blood lead was determined by graphite furnace atomic absorption spectrometry. We studied 88 adults (31 men and 57 women) from 18 to 60 years old. Pb-saliva levels varied from 0.05 to 4.4 microg/l, with a mean of 0.85 microg/l. Blood lead levels varied from 32.0 to 428.0 microg/l in men (mean 112.3 microg/l) and from 25.0 to 263.0 microg/l (mean 63.5 microg/l) in women. Corresponding Pb-Ps were 0.02-2.50 microg/l (mean 0.77 microg/l) and 0.03-1.6 microg/l (mean 0.42 microg/l) in men and women, respectively. A weak correlation was found between Log Pb-saliva and Log Pb-B (r=0.277, P<0.008), and between Log Pb-saliva and Log Pb-P (r=0.280, P=0.006). The Pb-saliva/Pb-P ratio ranged from 0.20 to 18.0. Age or gender does not affect Pb-saliva levels or Pb-saliva/Pb-P ratio. Taken together, these results suggest that salivary lead may not be used as a biomarker to diagnose lead exposure nor as a surrogate of plasma lead levels at least for low to moderately lead exposed population.
我们开展了一项研究，以评估腮腺唾液铅（Pb-唾液）水平作为血铅（Pb-B）或血浆铅水平（Pb-P）的替代指标用于诊断铅暴露的情况。在铅暴露人群中评估了这些生物标志物之间的关系。通过电感耦合等离子体质谱法测定Pb-唾液和Pb-P，而全血铅则通过石墨炉原子吸收光谱法测定。我们研究了88名年龄在18至60岁之间的成年人（31名男性和57名女性）。Pb-唾液水平在0.05至4.4微克/升之间，平均为0.85微克/升。男性血铅水平在32.0至428.0微克/升之间（平均112.3微克/升），女性血铅水平在25.0至263.0微克/升之间（平均63.5微克/升）。男性和女性相应的Pb-P分别为0.02 - 2.50微克/升（平均0.77微克/升）和0.03 - 1.6微克/升（平均0.42微克/升）。发现Log Pb-唾液与Log Pb-B之间存在弱相关性（r = 0.277，P < 0.008），Log Pb-唾液与Log Pb-P之间也存在弱相关性（r = 0.280，P = 0.006）。Pb-唾液/Pb-P比值在0.20至18.0之间。年龄或性别不影响Pb-唾液水平或Pb-唾液/Pb-P比值。综上所述，这些结果表明，至少对于低至中度铅暴露人群，唾液铅可能不能用作诊断铅暴露的生物标志物，也不能作为血浆铅水平的替代指标。
