Gorgulu Semih, Yagci Gokhan, Kaymakcioglu Nihat, Ozkara Murat, Kurt Bulent, Ozcan Ayhan, Kaya Omer, Sadir Serdar, Tufan Turgut
Department of General Surgery, Gulhane Military Medical Academy, 06018, Etlik, Ankara, Turkey.
Dig Dis Sci. 2006 Mar;51(3):480-7. doi: 10.1007/s10620-006-3159-2.
The aim of this study was to assess the efficiency of hyperbaric oxygen alone and in combination with 5-aminosalicylic acid in the acetic acid-induced colitis model, a well-known experimental model of inflammatory bowel disease in rats. Rats were randomly divided into five groups. In the noncolitis control group, rats were given isotonic saline, while in the other groups rats were treated by intracolonic administration of 4% acetic acid. In group 2, the untreated control group, no additional therapy was applied. In groups 3, 4, and 5 hyperbaric oxygen, 5-aminosalicylic acid. and 5-aminosalicylic acid + hyperbaric oxygen therapies were applied, respectively. Administration of acetic acid caused an inflammatory response in all animals. Histopathologic score was significantly higher in group 2 than in any other group. 5-Aminosalicylic acid and hyperbaric oxygen significantly decreased the histopathologic score (P < 0.05). Myeloperoxidase activity was also reduced significantly by 5-aminosalicylic acid (P < 0.05) but not by hyperbaric oxygen. The most prominent ameliorative effect, however, was seen in group 5 and the histopathologic score and myeloperoxidase activity were significantly lower than in groups 3 (P < 0.05) and 4 (P < 0.001). Hydroxyproline level also increased significantly in group 5, but not in groups 3 and 4 (P < 0.001). These findings indicate that hyperbaric oxygen therapy is effective in reducing the extent of colitis induced by acetic acid, although it is not as potent as 5-aminosalicylic acid. The combination of hyperbaric oxygen and 5-aminosalicylic acid, however, led to a much more prominent reduction in the severity of colitis. Hyperbaric oxygen may have a promising place in the treatment of inflammatory bowel disease.
本研究旨在评估单纯高压氧以及高压氧联合5-氨基水杨酸在乙酸诱导的结肠炎模型中的疗效，该模型是大鼠炎症性肠病的一种著名实验模型。大鼠被随机分为五组。在非结肠炎对照组中，大鼠给予等渗盐水，而在其他组中，大鼠通过结肠内给予4%乙酸进行处理。在第2组（未治疗对照组）中，未应用额外治疗。在第3组、第4组和第5组中，分别应用了高压氧、5-氨基水杨酸和5-氨基水杨酸 + 高压氧疗法。乙酸给药在所有动物中均引起炎症反应。第2组的组织病理学评分显著高于其他任何组。5-氨基水杨酸和高压氧显著降低了组织病理学评分（P < 0.05）。5-氨基水杨酸也显著降低了髓过氧化物酶活性（P < 0.05），但高压氧未降低。然而，最显著的改善效果出现在第5组，其组织病理学评分和髓过氧化物酶活性显著低于第3组（P < 0.05）和第4组（P < 0.001）。第5组的羟脯氨酸水平也显著升高，但第3组和第4组未升高（P < 0.001）。这些发现表明，高压氧疗法在减轻乙酸诱导的结肠炎程度方面是有效的，尽管其效果不如5-氨基水杨酸。然而，高压氧与5-氨基水杨酸联合使用导致结肠炎严重程度的降低更为显著。高压氧在炎症性肠病的治疗中可能具有广阔的应用前景。
