Holen Elisabeth, Bjørge Oddvin A, Jonsson Roland
Broegelmann Research Laboratory, University of Bergen, Norway.
Nutrition. 2006 Jan;22(1):90-6. doi: 10.1016/j.nut.2006.01.001.
The immune system is dependent on purines and pyrimidines as building blocks for DNA and RNA synthesis to enable rapid cell proliferation and protein synthesis. Emerging evidence suggests that dietary nucleotides optimize immune function. We investigated whether growth and function of human immune cells were affected by an exogenous source of nucleotides during specific antigen challenge.
Peripheral blood mononuclear cells from healthy individuals (n = 10) were stimulated with influenza virus antigen and either DNA sodium from fish soft roe (DNA), RNA from bakers yeast (Saccharomyces cerevisiae) (RNA), 2' deoxyadenosine 5'-monophosphate sodium (dAMP), 2' deoxycytidine 5'-monophosphate sodium (dCMP), 2' deoxyguanosine 5'-monophosphate sodium (dGMP), 2' deoxyuridine 5'-monophosphate sodium (dUMP) or thymidine sodium (TMP). Growth effects were ascertained by measuring the amount of tritium-labeled thymidine, incorporated into cell DNA. Cell function was measured by detection of IFN-gamma, TNF-alpha and IL-10 production.
Specific nucleotide derivatives alone did not affect the growth of healthy peripheral blood mononuclear cells. However, the nucleotide derivatives influenced immune cell growth and cytokine secretion when cocultured with specific antigen. DNA, RNA, dAMP, dCMP and dUMP increased influenza virus antigen induced immune cell proliferation. In contrast dGMP and TMP inhibited the antigen-induced growth response. RNA and dAMP cocultured with virus antigen significantly increased peripheral blood mononuclear cell secretion of IFN-gamma, IL-10 and TNF-alpha. DNA increased virus antigen-induced immune cell secretion of IFN-gamma only, whereas dUMP significantly increased secretion of IL-10 only. dGMP completely inhibited virus-triggered IFN-gamma secretion, whereas TMP did not change the virus induced secretion pattern of measured cytokines.
Nucleotide derivatives affect growth and function of specific virus antigen-stimulated human immune cells in vitro.
免疫系统依赖嘌呤和嘧啶作为DNA和RNA合成的构建基石，以实现快速的细胞增殖和蛋白质合成。新出现的证据表明，膳食核苷酸可优化免疫功能。我们研究了在特定抗原刺激期间，外源性核苷酸来源是否会影响人类免疫细胞的生长和功能。
用流感病毒抗原刺激来自健康个体（n = 10）的外周血单个核细胞，并分别加入鱼软籽DNA钠盐（DNA）、面包酵母（酿酒酵母）RNA（RNA）、2'-脱氧腺苷5'-磷酸钠盐（dAMP）、2'-脱氧胞苷5'-磷酸钠盐（dCMP）、2'-脱氧鸟苷5'-磷酸钠盐（dGMP）、2'-脱氧尿苷5'-磷酸钠盐（dUMP）或胸苷钠盐（TMP）。通过测量掺入细胞DNA中的氚标记胸苷的量来确定生长效应。通过检测IFN-γ、TNF-α和IL-10的产生来测量细胞功能。
单独的特定核苷酸衍生物不影响健康外周血单个核细胞的生长。然而，当与特定抗原共培养时，核苷酸衍生物会影响免疫细胞的生长和细胞因子分泌。DNA、RNA、dAMP、dCMP和dUMP可增加流感病毒抗原诱导的免疫细胞增殖。相比之下，dGMP和TMP抑制抗原诱导的生长反应。与病毒抗原共培养的RNA和dAMP显著增加外周血单个核细胞IFN-γ、IL-10和TNF-α的分泌。DNA仅增加病毒抗原诱导的免疫细胞IFN-γ的分泌，而dUMP仅显著增加IL-10的分泌。dGMP完全抑制病毒触发的IFN-γ分泌，而TMP未改变病毒诱导的所测细胞因子的分泌模式。
核苷酸衍生物在体外会影响特定病毒抗原刺激的人类免疫细胞的生长和功能。
