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蛋白酶水解结合发酵处理的海参内脏通过增强抗氧化能力、激活Nrf2/HO-1通路和改善细胞代谢保护Caco-2细胞免受氧化损伤。

Sea Cucumber Viscera Processed by Protease Hydrolysis Combined with Fermentation Protect Caco-2 Cells against Oxidative Damage via Enhancing Antioxidant Capacity, Activating Nrf2/HO-1 Pathway and Improving Cell Metabolism.

作者信息

Mi Rui, Fu Zhiyu, Jiang Jingwei, Gao Shan, Guan Xiaoyan, Wang Xuda, Zhou Zunchun

机构信息

Liaoning Ocean and Fisheries Science Research Institute, Liaoning Academy of Agricultural Sciences, Dalian 116024, China.

出版信息

Antioxidants (Basel). 2024 Aug 14;13(8):988. doi: 10.3390/antiox13080988.

DOI:10.3390/antiox13080988
PMID:39199234
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11351466/
Abstract

Excessive reactive oxygen species (ROS) may lead to oxidative damage and metabolic disorder. The pathogenesis of human bowel inflammation is closely related to oxidative damage of intestinal epithelial cells caused by ROS. This study aimed to explore the high-value utilization of the byproducts of sea cucumber in antioxidant food for colitis prevention. The technology of protease hydrolysis combined with fermentation was used to obtain fermented sea cucumber viscera protease hydrolysates (FSVHs). The results revealed that FSVH could enhance antioxidant capacity and alleviate oxidative damage and apoptosis by activating the Nrf2/HO-1 pathway and triggering the self-protection immune mechanisms. Moreover, the FSVH supplementation could upregulate antioxidant-related metabolic pathways of Caco-2 cells such as glutathione metabolism, confirming the enhanced antioxidant capacity of damaged cells. In summary, FSVH could exert protective effects on Caco-2 cells in response to oxidative damage, providing a promising prospect for sea cucumber resource utilization and colitis prevention.

摘要

过量的活性氧（ROS）可能导致氧化损伤和代谢紊乱。人类肠道炎症的发病机制与ROS引起的肠道上皮细胞氧化损伤密切相关。本研究旨在探索海参副产物在预防结肠炎的抗氧化食品中的高值利用。采用蛋白酶水解结合发酵技术制备发酵海参内脏蛋白酶水解物（FSVHs）。结果表明，FSVH可通过激活Nrf2/HO-1途径和触发自我保护免疫机制来增强抗氧化能力，减轻氧化损伤和细胞凋亡。此外，补充FSVH可上调Caco-2细胞抗氧化相关代谢途径，如谷胱甘肽代谢，证实受损细胞的抗氧化能力增强。综上所述，FSVH对氧化损伤的Caco-2细胞具有保护作用，为海参资源利用和结肠炎预防提供了广阔前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5905/11351466/79e044b6e91b/antioxidants-13-00988-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5905/11351466/1f9ab479bdc8/antioxidants-13-00988-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5905/11351466/058ecb1e9273/antioxidants-13-00988-g006.jpg
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