利巴韦林对回忆抗原和植物血凝素刺激的外周血单个核细胞细胞因子产生的影响。（利巴韦林对细胞因子产生的抑制作用）

Effects of ribavirin on cytokine production of recall antigens and phytohemaglutinin-stimulated peripheral blood mononuclear cells. (Inhibitory effects of ribavirin on cytokine production).

作者信息

Sookoian Silvia, Castaño Gustavo, Flichman Diego, Cello Jerónimo

机构信息

Gastroenterology Section, Internal Medicine Department, Hospital JM Penna, GCBA.

出版信息

Ann Hepatol. 2004 Jul-Sep;3(3):104-7. doi: 10.1016/S1665-2681(19)32102-7.

DOI:10.1016/S1665-2681(19)32102-7

PMID:15505595

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7194803/

Abstract

AIM

To evaluate the effects of ribavirin on cytokine production of recall antigen and PHA-stimulated PBMC obtained from healthy individuals.

MATERIALS AND METHODS

PBMC were challenged with tetanus toxoid (5 microg/mL) and PHA (10 microg/mL) in absence or presence of ribavirin at different concentrations (1, 10 and 100 (microM). Parallel sets of wells containing PBMC exposed to medium alone were used as negative controls. On day 3 after initiation of the cultures, IL-2, IFN-gamma, IL-4, IL-10, TNF-alpha content were determined in supernatants of PBMC from the different individuals.

RESULTS

The effects of ribavirin on cytokine released by human PBMC in response to PHA and TT showed a great variation among individuals. No significant changes were observed between 1-10 microM concentrations in the production of TNF-alpha, IFN-gamma and IL-10 by both PHA and TT-stimulated PBMC. Ribavirin inhibited TNF-alpha, IFN-gamma, and IL-10 in both PHA and TT-stimulated PBMC at 100 microM (p <0.05). At this concentration, ribavirin induced an increase of 124% in the production of IL-2 by PHA-stimulated PBMC (p <0.05).

CONCLUSIONS

The present data suggest that ribavirin may cause diverse effects on immunoregulatory cytokine secretion with changes in the Th1/Th2 balance.

摘要

目的

评估利巴韦林对从健康个体获得的回忆抗原和PHA刺激的外周血单个核细胞（PBMC）细胞因子产生的影响。

材料与方法

在不存在或存在不同浓度（1、10和100μM）利巴韦林的情况下，用破伤风类毒素（5μg/mL）和PHA（10μg/mL）刺激PBMC。将含有仅暴露于培养基的PBMC的平行孔板用作阴性对照。在培养开始后的第3天，测定来自不同个体的PBMC上清液中的IL-2、IFN-γ、IL-4、IL-10、TNF-α含量。

结果

利巴韦林对人PBMC响应PHA和破伤风类毒素释放的细胞因子的影响在个体间存在很大差异。在1-10μM浓度之间，PHA和破伤风类毒素刺激的PBMC产生的TNF-α、IFN-γ和IL-10没有观察到显著变化。在100μM时，利巴韦林抑制PHA和破伤风类毒素刺激的PBMC中的TNF-α、IFN-γ和IL-10（p<0.05）。在此浓度下，利巴韦林使PHA刺激的PBMC中IL-2的产生增加了124%（p<0.05）。