Sandgren M E, Brönnegärd M, DeLuca H F
Department of Biochemistry, University of Wisconsin-Madison 53706.
Biochem Biophys Res Commun. 1991 Dec 16;181(2):611-6. doi: 10.1016/0006-291x(91)91234-4.
Tissue distribution of 1,25-dihydroxyvitamin D3 receptors was studied in male rats using a quantitative immunoradiometric assay. Extracts were prepared from 16 different rat tissues and assayed for 1,25-dihydroxyvitamin D3 receptor. Measurable levels of receptor were detected in intestine, stomach, kidney, bone thyroid/parathyroid, skin, liver, spleen, heart and lung. The highest levels were found in the proximal small intestine and colon, containing over 1000 fmol/mg total protein, while ileum and kidney contained one-half and one-fourth of this amount, respectively. Other parts of the vitamin D endocrine system, including bone, thyroid/parathyroid and skin, contained moderate levels of receptor of 40 to 80 fmol/mg, while lung, heart, stomach, spleen and liver had levels at or below 20 fmol/mg. No 1,25-dihydroxyvitamin D3 receptor was detected in cerebrum, cerebellum or skeletal muscle. The data support a wide-spread role for 1,25-dihydroxyvitamin D3 on cellular processes and suggest a more important role for vitamin D in colon.
利用定量免疫放射分析方法，对雄性大鼠体内1,25 - 二羟基维生素D3受体的组织分布进行了研究。从16种不同的大鼠组织中提取样本，并对1,25 - 二羟基维生素D3受体进行检测。在小肠、胃、肾脏、骨骼、甲状腺/甲状旁腺、皮肤、肝脏、脾脏、心脏和肺中检测到了可测量水平的受体。最高水平出现在近端小肠和结肠，每毫克总蛋白中含量超过1000飞摩尔，而回肠和肾脏中的含量分别为其一半和四分之一。维生素D内分泌系统的其他部分，包括骨骼、甲状腺/甲状旁腺和皮肤，受体含量中等，为每毫克40至80飞摩尔，而肺、心脏、胃、脾脏和肝脏中的含量等于或低于每毫克20飞摩尔。在大脑、小脑或骨骼肌中未检测到1,25 - 二羟基维生素D3受体。这些数据支持了1,25 - 二羟基维生素D3在细胞过程中具有广泛作用的观点，并表明维生素D在结肠中发挥着更重要的作用。
