地塞米松对大鼠睾丸缺血再灌注损伤后对侧睾丸生殖细胞凋亡的影响。
Effect of dexamethasone on germ cell apoptosis in the contralateral testis after testicular ischemia-reperfusion injury in the rat.
作者信息
Mogilner Jorge G, Elenberg Yigal, Lurie Michael, Shiloni Eitan, Coran Arnold G, Sukhotnik Igor
机构信息
Department of Pediatric Surgery, Bruce Rappaport Faculty of Medicine, Technion, Bnai Zion Medical Center, Haifa, Israel.
出版信息
Fertil Steril. 2006 Apr;85 Suppl 1:1111-7. doi: 10.1016/j.fertnstert.2005.10.021.
OBJECTIVE
To evaluate the effect of dexamethasone on spermatogenesis and germ cell apoptosis in the ipsilateral and contralateral testis after testicular ischemia-reperfusion (IR) in rats.
DESIGN
Laboratory study.
SETTING
Medical research laboratory in a university setting.
ANIMAL(S): Forty adult Sprague-Dawley rats weighing 250-280 g.
INTERVENTION(S): Testicular IR, treatment with dexamethasone (10 mg per kilogram of body weight).
MAIN OUTCOME MEASURE(S): Testicular germ cell apoptosis was assessed by deoxyuridine nick-end labeling immunohistochemical assay.
RESULT(S): Testicular ischemia in rats led to histological damage in the ipsilateral testis. In the contralateral testis, minimal damage was observed. Germ cell apoptosis in both the ischemic and the contralateral testis increased significantly after IR. Treatment with dexamethasone did not change germ cell apoptosis in ischemic testis but decreased germ cell apoptosis in the contralateral testis.
CONCLUSION(S): Glucocorticoids may be beneficial for spermatogenesis after testicular IR by decreasing germ cell apoptosis in the contralateral testis.
目的
评估地塞米松对大鼠睾丸缺血再灌注（IR）后同侧和对侧睾丸生精及生殖细胞凋亡的影响。
设计
实验室研究。
单位
大学环境中的医学研究实验室。
动物
40只体重250 - 280克的成年Sprague-Dawley大鼠。
干预措施
睾丸IR，用地塞米松治疗（每千克体重10毫克）。
主要观察指标
采用脱氧尿苷缺口末端标记免疫组化法评估睾丸生殖细胞凋亡。
结果
大鼠睾丸缺血导致同侧睾丸组织学损伤。对侧睾丸观察到轻微损伤。IR后，缺血睾丸和对侧睾丸的生殖细胞凋亡均显著增加。地塞米松治疗未改变缺血睾丸的生殖细胞凋亡，但降低了对侧睾丸的生殖细胞凋亡。
结论
糖皮质激素可能通过减少对侧睾丸生殖细胞凋亡，对睾丸IR后的生精有益。
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