Department of Urology, University of Health Sciences, Ankara Training and Research Hospital, Ankara-Turkey.
Department of Biochemistry, University of Health Sciences, Ankara Training and Research Hospital, Ankara-Turkey.
Ulus Travma Acil Cerrahi Derg. 2022 Jun;28(6):730-735. doi: 10.14744/tjtes.2021.01361.
The aim of the present study is to investigate the efficiency of roflumilast and ibuprofen in an experimental rat testicular ischemia reperfusion injury model in the light of histological and biochemical data.
A total of 32 prepubertal male rats were randomly divided into four groups as G1: Control Group (testicular torsion/detorsion + saline (0.9% of 2 ml) was applied). G2: Sham Group only right scrotal incision was performed; G3: Ibuprofen Group (tes-ticular torsion/detorsion + ibuprofen administration); and G4 Roflumilast Group (testicular torsion/detorsion + roflumilast adminis-tration). Oxidative markers such as malondialdehyde (MDA), myeloperoxidase (MPO), total sulfhydryl (TSH), and nitrite (NO) levels as well as histopathological changes were analyzed.
Tissue MPO, MDA, and NO levels were significantly higher and TSH levels significantly lower in control group compared to sham group (p<0.001). The histopathologic scores of drug groups (Groups 3 and 4) were significantly lower than group 1 (p<0.001). In comparison of Group 3 and Group 4 with each other, the mean values of MPO and MDA were statistically significantly lower in Group 4 (p<0.001). A higher mean value of TSH was found in Group 3 without statistically significance (p=0.32). There was also an insignificant decrease in mean NO values of Group 3 compared to Group 4 (p=0.44). In comparison of drug groups, Group 4 had statistically insignificant better scores.
Our results indicate that administrating ibuprofen and roflumilast reduced testicular ischemia reperfusion injury in rat testis torsion model. In comparison, roflumilast is found to be more beneficial.
本研究旨在根据组织学和生化数据，探讨罗氟司特和布洛芬在实验性大鼠睾丸缺血再灌注损伤模型中的疗效。
将 32 只未成熟雄性大鼠随机分为 4 组：G1 组（睾丸扭转/复位+生理盐水（2ml0.9%））；G2 组（仅行右侧阴囊切开术）；G3 组（布洛芬组，睾丸扭转/复位+布洛芬给药）；G4 组（罗氟司特组，睾丸扭转/复位+罗氟司特给药）。分析氧化标志物如丙二醛（MDA）、髓过氧化物酶（MPO）、总巯基（TSH）和亚硝酸盐（NO）水平以及组织病理学变化。
与 sham 组相比，对照组（G1 组）组织 MPO、MDA 和 NO 水平显著升高，TSH 水平显著降低（p<0.001）。与 G1 组相比，药物组（G3 和 G4 组）的组织病理评分明显降低（p<0.001）。与 G3 组相比，G4 组的 MPO 和 MDA 均值明显降低（p<0.001）。G3 组 TSH 均值较高，但无统计学意义（p=0.32）。与 G4 组相比，G3 组的 NO 均值也略有下降，但无统计学意义（p=0.44）。与药物组相比，G4 组的评分略有提高，但无统计学意义。
我们的研究结果表明，在大鼠睾丸扭转模型中，给予布洛芬和罗氟司特均可减轻睾丸缺血再灌注损伤。相比之下，罗氟司特的效果更显著。
