Qiu Hongdeng, Jiang Shengxiang, Liu Xia, Zhao Liang
Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
J Chromatogr A. 2006 May 26;1116(1-2):46-50. doi: 10.1016/j.chroma.2006.03.016. Epub 2006 Apr 17.
A new imidazolium anion-exchange phase immobilized on silica is synthesized. HPLC separations of common inorganic anions (IO3-, Cl-, NO2-, Br-, NO3-, I-, SCN-) have been performed using a HPLC column (200 mm x 4.6 mm I.D.) packed with this stationary phase, with a phosphate buffer solution as the mobile phase and UV detection at 200 nm. The effects of pH and concentration of eluent on the separation of anions have been studied. Chromatographic parameters are calculated and the results show that the new stationary phase is of significant potential for the analysis of these anions. Successful separations of some ordinary organic anions have also been achieved with the said stationary phase. Meaningfully, organic and inorganic anions can be determined simultaneously and satisfactorily with several neutral compounds using the column. The separation of some organic compounds including hydroxybenzenes, bases and amines by this stationary phase with only water as the eluent has been investigated.
合成了一种固定在硅胶上的新型咪唑鎓阴离子交换相。使用填充有该固定相的高效液相色谱柱（200 mm×4.6 mm内径），以磷酸盐缓冲溶液为流动相，在200 nm处进行紫外检测，对常见无机阴离子（IO3-、Cl-、NO2-、Br-、NO3-、I-、SCN-）进行了高效液相色谱分离。研究了pH值和洗脱液浓度对阴离子分离的影响。计算了色谱参数，结果表明该新型固定相对这些阴离子的分析具有很大潜力。使用该固定相也成功实现了一些普通有机阴离子的分离。有意义的是，使用该色谱柱可以同时令人满意地测定有机和无机阴离子以及几种中性化合物。研究了仅以水为洗脱液时，该固定相对包括羟基苯、碱和胺在内的一些有机化合物的分离。
