Esteban J, Fernández Roblas R, García Cía J I, Zamora N, Ortiz A
Department of Clinical Microbiology, Fundación Jiménez Díaz, Av. Reyes Católicos 2, 28040-Madrid, Spain.
J Infect. 2007 Feb;54(2):135-45. doi: 10.1016/j.jinf.2006.02.017. Epub 2006 Apr 17.
To study the clinical significance and epidemiology of Non-pigmented rapidly growing mycobacteria (NPRGM) during a 13-year period.
We performed a retrospective study of patients with isolates of NPRGM to evaluate their clinical significance. We also analyzed the strains using Randomly Amplified Polymorphic DNA (RAPD) analysis to evaluate the relationship between strains.
Between 1990 and 2003, 65 patients had an isolate of NPRGM. Twenty of them were considered significant (19 cases) or doubtful (1 case). Many cases were skin and soft tissue infections. Six cases were foreign-body related. All the patients recovered with antibiotic therapy and removal of the foreign body. All the patients were apparently unrelated, despite 56.9% of the isolates were detected between 1995 and 1997. RAPD analysis was performed on 43 strains, and showed only a cluster of two Mycobacterium chelonae isolates. Both of them were related with contamination of a laboratory reactive, and were considered non-significant.
In our hospital, almost one-third of the isolates of NPRGM were significant, being this percentage higher for skin and soft tissue isolates. Patients were cured with antibiotic therapy, but the removal of foreign bodies appeared to be necessary for a good outcome. A minor pseudo-outbreak was detected. No predominant strain was detected.
研究13年间非色素快速生长分枝杆菌（NPRGM）的临床意义及流行病学特征。
我们对分离出NPRGM的患者进行了一项回顾性研究，以评估其临床意义。我们还使用随机扩增多态性DNA（RAPD）分析对菌株进行分析，以评估菌株之间的关系。
1990年至2003年期间，65例患者分离出NPRGM。其中20例被认为具有显著性（19例）或可疑（1例）。许多病例为皮肤和软组织感染。6例与异物相关。所有患者经抗生素治疗及取出异物后均康复。尽管56.9%的分离株是在1995年至1997年期间检测到的，但所有患者显然无关联。对43株菌株进行了RAPD分析，仅显示出两株龟分枝杆菌分离株的一个聚类。它们均与实验室反应剂污染有关，被认为无显著性。
在我们医院，几乎三分之一的NPRGM分离株具有显著性，皮肤和软组织分离株的这一比例更高。患者经抗生素治疗后治愈，但取出异物似乎对良好预后是必要的。检测到一次小规模的假暴发。未检测到优势菌株。
