Esteban Jaime, Martín-de-Hijas Nieves Z, Kinnari Teemu J, Ayala Guillermo, Fernández-Roblas Ricardo, Gadea Ignacio
Department of Clinical Microbiology, Fundación Jiménez Díaz-UTE, Madrid, Spain.
BMC Microbiol. 2008 Oct 17;8:184. doi: 10.1186/1471-2180-8-184.
A study to evaluate the biofilm-development ability in three different media (Middlebrook 7H9, sterile tap water and PBS-5% glucose) was performed with 19 collection strains from 15 different species on non-pigmented rapidly growing mycobacteria (NPRGM). A microtiter plate assay was developed to evaluate the percentage of covered surface of the microtiter plate wells in different days from day 1 to day 69.
All strains were able to develop biofilm in all the tested media. Middlebrook 7H9 showed the fastest growth, followed by sterile tap water and PBS-5% glucose. A sigmoid growth curve was detected in all the strains both in Middlebrook 7H9 and in sterile tap water. A difference could be detected for Mycobacterium abscessus in tap water, where it showed faster growth than all the other strains.
Biofilm development seems to be a property of all the species of NPRGM and it depends on the nutrients present in the medium. The microtiter plate assay described here is a useful tool to evaluate differences in biofilm development among the different species of rapidly growing mycobacteria.
对来自15个不同物种的19株非色素快速生长分枝杆菌（NPRGM）的菌株进行了一项研究，以评估其在三种不同培养基（Middlebrook 7H9、无菌自来水和含5%葡萄糖的磷酸盐缓冲液）中形成生物膜的能力。开发了一种微量滴定板测定法，以评估从第1天到第69天不同时间微量滴定板孔表面被覆盖的百分比。
所有菌株在所有测试培养基中均能形成生物膜。Middlebrook 7H9生长最快，其次是无菌自来水和含5%葡萄糖的磷酸盐缓冲液。在Middlebrook 7H9和无菌自来水中，所有菌株均检测到S形生长曲线。脓肿分枝杆菌在自来水中生长速度比其他所有菌株都快，可检测到差异。
生物膜形成似乎是所有NPRGM物种的特性，并且它取决于培养基中存在的营养物质。本文所述的微量滴定板测定法是评估不同快速生长分枝杆菌物种之间生物膜形成差异的有用工具。
