Guillon Stéphanie, Trémouillaux-Guiller Jocelyne, Pati Pratap Kumar, Rideau Marc, Gantet Pascal
UPRES EA 2106 'Biomolécules et Biotechnologies Végétales', Université François Rabelais, UFR des Sciences Pharmaceutiques, Parc de Grandmont, 37200 Tours, France.
Curr Opin Plant Biol. 2006 Jun;9(3):341-6. doi: 10.1016/j.pbi.2006.03.008. Epub 2006 Apr 17.
High stability of the production of secondary metabolites is an interesting characteristic of hairy root cultures. For 25 years, hairy roots have been investigated as a biological system for the production of valuable compounds from medicinal plants. A better understanding of the molecular mechanism of hairy root development, which is based on the transfer of Agrobacterium rhizogenes T-DNA into the plant genome, has facilitated its increasing use in metabolic engineering. Hairy roots can also produce recombinant proteins from transgenic roots, and thereby hold immense potential for the pharmaceutical industry. In addition, hairy roots offer promise for phytoremediation because of their abundant neoplastic root proliferation. Recent progress in the scaling-up of hairy root cultures is making this system an attractive tool for industrial processes.
次生代谢产物生产的高稳定性是毛状根培养的一个有趣特性。25年来，毛状根一直被作为一种从药用植物中生产有价值化合物的生物系统进行研究。对基于发根农杆菌T-DNA转移到植物基因组的毛状根发育分子机制的更好理解，促进了其在代谢工程中的日益广泛应用。毛状根还可以从转基因根中产生重组蛋白，因此在制药工业中具有巨大潜力。此外，由于其大量的肿瘤性根增殖，毛状根在植物修复方面也有前景。毛状根培养放大方面的最新进展使该系统成为工业生产过程的一个有吸引力的工具。
