Georgiev Milen I, Pavlov Atanas I, Bley Thomas
Institute of Food Technology and Bioprocess Engineering, Dresden University of Technology, 01069 Dresden, Germany.
Appl Microbiol Biotechnol. 2007 Apr;74(6):1175-85. doi: 10.1007/s00253-007-0856-5. Epub 2007 Feb 10.
"Hairy root" systems, obtained by transforming plant tissues with the "natural genetic engineer" Agrobacterium rhizogenes, have been known for more than three decades. To date, hairy root cultures have been obtained from more than 100 plant species, including several endangered medicinal plants, affording opportunities to produce important phytochemicals and proteins in eco-friendly conditions. Diverse strategies can be applied to improve the yields of desired metabolites and to produce recombinant proteins. Furthermore, recent advances in bioreactor design and construction allow hairy root-based technologies to be scaled up while maintaining their biosynthetic potential. This review highlights recent progress in the field and outlines future prospects for exploiting the potential utility of hairy root cultures as "chemical factories" for producing bioactive substances.
通过用“天然基因工程师”发根农杆菌转化植物组织获得的“毛状根”系统，已为人所知超过三十年。迄今为止，已从100多种植物中获得了毛状根培养物，包括几种濒危药用植物，这为在生态友好的条件下生产重要的植物化学物质和蛋白质提供了机会。可以应用多种策略来提高所需代谢物的产量并生产重组蛋白。此外，生物反应器设计和构建方面的最新进展使基于毛状根的技术能够扩大规模，同时保持其生物合成潜力。本综述重点介绍了该领域的最新进展，并概述了利用毛状根培养物作为生产生物活性物质的“化学工厂”的潜在用途的未来前景。
