Vercruysse J, Geurden T, Peelaers I
Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Vet Rec. 2006 Apr 15;158(15):506-8. doi: 10.1136/vr.158.15.506.
Samples of ear scrapings, serum and diaphragmatic muscle were collected from 271 fattening pigs at the slaughterhouse. The scrapings were examined for the presence of mites, and tests for specific antibodies to Sarcoptes scabiei var suis in the serum and meat juice were made with an experimental ELISA. The cut-off value for the meat-juice ELISA was estimated at an optical density of 0.5 by receiver operating characteristic curve analysis, on the basis of the cut-off value for the serum ELISA of 0.4. The results of the three tests were used in a Bayesian model to estimate the characteristics of each test. The specificity of the tests of the ear scrapings was considered to be 1 and their sensitivity was estimated by Bayesian analysis to be 0.86, with a 95 per cent confidence interval (CI) of 0.73 to 0.99. The sensitivity of the meat juice ELISA (0.71, 95 per cent CI 0.6 to 0.8) and its specificity (0.77, 95 per cent CI 0.66 to 0.89) were comparable with the sensitivity (0.73, 95 per cent CI 0.6 to 0.8) and specificity (0.81, 95 per cent CI 0.69 to 0.95) of the serum ELISA.
在屠宰场从271头育肥猪身上采集了耳刮屑、血清和膈肌样本。检查刮屑中是否存在螨虫，并采用实验性酶联免疫吸附测定法（ELISA）检测血清和肉汁中针对猪疥螨变种的特异性抗体。根据血清ELISA的临界值0.4，通过受试者工作特征曲线分析，将肉汁ELISA的临界值估计为光密度0.5。三项检测结果用于贝叶斯模型，以评估每项检测的特性。耳刮屑检测的特异性被认为是1，通过贝叶斯分析估计其敏感性为0.86，95%置信区间（CI）为0.73至0.99。肉汁ELISA的敏感性（0.71，95%CI 0.6至0.8）及其特异性（0.77，95%CI 0.66至0.89）与血清ELISA的敏感性（0.73，95%CI 0.6至0.8）和特异性（0.81，95%CI 0.69至0.95）相当。
