Hollanders W, Vercruysse J, Raes S, Bornstein S
Faculty of Veterinary Medicine, Department of Parasitology, Merelbeke, Belgium.
Vet Parasitol. 1997 Apr;69(1-2):117-23. doi: 10.1016/s0304-4017(96)01117-x.
An enzyme-linked immunosorbent assay (ELISA) using an extract from Sarcoptes scabiei var. culpes as antigen was evaluated for its usefulness in monitoring antibodies to S. scabiei var. suis in naturally infected pigs. Five hundred and fifty-eight serum samples from certified Sarcoptes-free pigs (120 from weaned piglets, 218 from fatteners and 220 from adult sows) and 94 samples from certified Sarcoptes-infected pigs (35 from weaned piglets, 45 from fatteners and 14 from adult sows) were examined. The cut-off optical density (OD) values, considered to reflect the presence of antibodies to S. scabiei var. suis, were determined to be 0.059, 0.213 and 0.374 for weaners, fatteners and sows, respectively. The specificity of the ELISA was high (> or = 98%) for all age groups. Sensitivity was highest in the piglets (80%), followed by fatteners (78%) and sows (50%). These results indicate that an ELISA may be a simple and valuable alternative to monitor S. seabiei var. suis infections in naturally infected weaners and fatteners.
评估了一种以猪疥螨提取物为抗原的酶联免疫吸附测定（ELISA）在监测自然感染猪体内猪疥螨抗体方面的实用性。检测了来自经认证无疥螨猪的558份血清样本（120份来自断奶仔猪，218份来自育肥猪，220份来自成年母猪）以及来自经认证感染疥螨猪的94份样本（35份来自断奶仔猪，45份来自育肥猪，14份来自成年母猪）。断奶仔猪、育肥猪和母猪被认为反映猪疥螨抗体存在的临界光密度（OD）值分别确定为0.059、0.213和0.374。ELISA对所有年龄组的特异性都很高（≥98%）。敏感性在仔猪中最高（80%），其次是育肥猪（78%）和母猪（50%）。这些结果表明，ELISA可能是监测自然感染的断奶仔猪和育肥猪中猪疥螨感染的一种简单且有价值的替代方法。
