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当在模型膜和活细胞中对相位敏感染料二-4-ANEPPDHQ进行成像时，荧光寿命成像可提供增强的对比度。

Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells.

作者信息

Owen Dylan M, Lanigan Peter M P, Dunsby Christopher, Munro Ian, Grant David, Neil Mark A A, French Paul M W, Magee Anthony I

机构信息

Chemical Biology Centre, Department of Physics, Imperial College London, London, United Kingdom.

出版信息

Biophys J. 2006 Jun 1;90(11):L80-2. doi: 10.1529/biophysj.106.084673. Epub 2006 Apr 14.

DOI:10.1529/biophysj.106.084673
PMID:16617080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1459501/
Abstract

We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-beta-cyclodextrin.

摘要

我们将荧光寿命成像技术应用于模型膜和活细胞中的膜相传感染料二-4-ANEPPDHQ。我们发现，与先前报道的60纳米光谱位移相比，液体无序相和液体有序相之间1700皮秒的寿命位移提供了更大的对比度。通过观察膜有序性的温度依赖性以及使用甲基-β-环糊精消耗胆固醇，证实了富含胆固醇的膜微区的检测。

相似文献

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Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells.当在模型膜和活细胞中对相位敏感染料二-4-ANEPPDHQ进行成像时，荧光寿命成像可提供增强的对比度。
Biophys J. 2006 Jun 1;90(11):L80-2. doi: 10.1529/biophysj.106.084673. Epub 2006 Apr 14.
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本文引用的文献

1
Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.富含胆固醇的脂质结构域可用二-4-ANEPPDHQ通过线性和非线性光学进行可视化。
Biophys J. 2005 Jul;89(1):L04-6. doi: 10.1529/biophysj.105.064816. Epub 2005 May 6.
2
Integrin-dependent interaction of lipid rafts with the actin cytoskeleton in activated human platelets.活化的人血小板中脂筏与肌动蛋白细胞骨架的整合素依赖性相互作用。
J Cell Sci. 2005 Feb 15;118(Pt 4):759-69. doi: 10.1242/jcs.01648. Epub 2005 Jan 25.
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Novel naphthylstyryl-pyridium potentiometric dyes offer advantages for neural network analysis.新型萘基苯乙烯基吡啶电位染料为神经网络分析提供了优势。
J Neurosci Methods. 2004 Apr 30;134(2):179-90. doi: 10.1016/j.jneumeth.2003.11.011.
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Visualizing lipid structure and raft domains in living cells with two-photon microscopy.利用双光子显微镜观察活细胞中的脂质结构和筏结构域。
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15554-9. doi: 10.1073/pnas.2534386100. Epub 2003 Dec 12.
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Sphingomyelin/phosphatidylcholine/cholesterol phase diagram: boundaries and composition of lipid rafts.鞘磷脂/磷脂酰胆碱/胆固醇相图：脂筏的边界与组成
Biophys J. 2003 Oct;85(4):2406-16. doi: 10.1016/S0006-3495(03)74664-5.
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