当在模型膜和活细胞中对相位敏感染料二-4-ANEPPDHQ进行成像时，荧光寿命成像可提供增强的对比度。
Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells.
作者信息
Owen Dylan M, Lanigan Peter M P, Dunsby Christopher, Munro Ian, Grant David, Neil Mark A A, French Paul M W, Magee Anthony I
机构信息
Chemical Biology Centre, Department of Physics, Imperial College London, London, United Kingdom.
出版信息
Biophys J. 2006 Jun 1;90(11):L80-2. doi: 10.1529/biophysj.106.084673. Epub 2006 Apr 14.
Abstract
We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-beta-cyclodextrin.
摘要
我们将荧光寿命成像技术应用于模型膜和活细胞中的膜相传感染料二-4-ANEPPDHQ。我们发现，与先前报道的60纳米光谱位移相比，液体无序相和液体有序相之间1700皮秒的寿命位移提供了更大的对比度。通过观察膜有序性的温度依赖性以及使用甲基-β-环糊精消耗胆固醇，证实了富含胆固醇的膜微区的检测。
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本文引用的文献
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Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.富含胆固醇的脂质结构域可用二-4-ANEPPDHQ通过线性和非线性光学进行可视化。
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