Jin Lei, Millard Andrew C, Wuskell Joseph P, Clark Heather A, Loew Leslie M
Department of Cell Biology and Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, Connecticut 06030-1507, USA.
Biophys J. 2005 Jul;89(1):L04-6. doi: 10.1529/biophysj.105.064816. Epub 2005 May 6.
We present a membrane-staining dye, di-4-ANEPPDHQ, which differentiates liquid-ordered phases from liquid-disordered phases coexisting in model membranes under both linear and nonlinear microscopies. The dye's fluorescence emission spectrum is blue-shifted 60 nm in liquid-ordered phases compared with liquid-disordered phases, and shows strong second harmonic generation in the liquid-disordered phase compared with the liquid-ordered phase. The ease of staining and the ability of this single dye to detect both phases, should lead to broad applications in biophysical studies of lipid domains in model membranes and cells.
我们展示了一种膜染色染料二-4-ANEPPDHQ，它能在线性和非线性显微镜下区分模型膜中共存的液相有序相和液相无序相。与液相无序相相比，该染料在液相有序相中的荧光发射光谱蓝移了60纳米，并且与液相有序相相比，在液相无序相中表现出强烈的二次谐波产生。这种单一染料易于染色且能够检测这两种相，这将使其在模型膜和细胞中脂质结构域的生物物理研究中得到广泛应用。
