Rubin L L, Hall D E, Porter S, Barbu K, Cannon C, Horner H C, Janatpour M, Liaw C W, Manning K, Morales J
Athena Neurosciences, Inc., South San Francisco, California 94080.
J Cell Biol. 1991 Dec;115(6):1725-35. doi: 10.1083/jcb.115.6.1725.
Endothelial cells that make up brain capillaries and constitute the blood-brain barrier become different from peripheral endothelial cells in response to inductive factors found in the nervous system. We have established a cell culture model of the blood-brain barrier by treating brain endothelial cells with a combination of astrocyte-conditioned medium and agents that elevate intracellular cAMP. These cells form high resistance tight junctions and exhibit low rates of paracellular leakage and fluid-phase endocytosis. They also undergo a dramatic structural reorganization as they form tight junctions. Results from these studies suggest modes of manipulating the permeability of the blood-brain barrier, potentially providing the basis for increasing the penetration of drugs into the central nervous system.
构成脑毛细血管并形成血脑屏障的内皮细胞，会因神经系统中发现的诱导因子而与外周内皮细胞有所不同。我们通过用星形胶质细胞条件培养基和提高细胞内cAMP的试剂处理脑内皮细胞，建立了血脑屏障的细胞培养模型。这些细胞形成高电阻紧密连接，细胞旁渗漏率和液相内吞率较低。它们在形成紧密连接时还会经历显著的结构重组。这些研究结果提示了操纵血脑屏障通透性的方式，有可能为增加药物进入中枢神经系统的渗透率提供依据。
