Kuljanac Ilko, Milavec-Puretić Visnja
Department of Dermatovenerology, General Hospital Karlovac, Karlovac, Croatia.
Coll Antropol. 2006 Mar;30(1):181-3.
The aim of this study was to evaluate the role of Dermatophagoides pteronyssinus (Dp) in atopic dermatitis patients, using atopy patch test (APT) with Dp (extract 1). Twenty patients (males (m) = 9, females (f) = 11, mean age = 46.0 years, range = 19-78 years) with atopic dermatitis were involved in this study. The control group consisted of seventeen healthy subjects (m = 7, f = 10, mean age = 48.3, range = 24-64 years), with no personal or family history and no signs of atopy. Total IgE, specific IgE and a skin prick test were done for all subjects involved in this study. The atopy patch tests were performed with Dp (extract 1) in: 3,000, 10,000, 20,000 and 30,000 biological units per ml (BU/ml) concentrations using glycerol as medium. The total IgE was significantly higher in atopic dermatitis (AD) patients than in a control group with (p < 0.05). After the tests six of twenty patients (30%) had positive APT results in the last two concentrations (20,000 and 30,000 BU/ml). However, all the results were positive after 48 h (and 72 hours), while no positive results were recorded in the control subjects. According to our study, APT with Dp 1 in 20,000 BU/ml and reading time 48 h and 72 hours is to be recommended. The results suggest that APT may detect the trigger factor (Dp) in AD patients.
本研究旨在通过使用含粉尘螨（提取物1）的特应性斑贴试验（APT），评估粉尘螨在特应性皮炎患者中的作用。20例特应性皮炎患者（男性9例，女性11例，平均年龄46.0岁，范围19 - 78岁）参与了本研究。对照组由17名健康受试者组成（男性7例，女性10例，平均年龄48.3岁，范围24 - 64岁），他们无个人或家族过敏史且无特应性体征。对本研究中的所有受试者进行了总IgE、特异性IgE检测及皮肤点刺试验。使用甘油作为介质，以每毫升3000、10000、20000和30000生物单位（BU/ml）的浓度对粉尘螨（提取物1）进行特应性斑贴试验。特应性皮炎（AD）患者的总IgE显著高于对照组（p < 0.05）。测试后，20例患者中有6例（30%）在最后两个浓度（20000和30000 BU/ml）时APT结果为阳性。然而，所有结果在48小时（和72小时）后均为阳性，而对照组受试者未记录到阳性结果。根据我们的研究，建议使用浓度为20000 BU/ml的粉尘螨1进行APT，读取时间为48小时和72小时。结果表明，APT可能检测出AD患者中的触发因素（粉尘螨）。
